Method for ultrasonic acid extraction of sulfated polysaccharide from gracilaria tenuistipitata

Abstract

The invention discloses a method for ultrasonic acid extraction of sulfated polysaccharide from gracilaria tenuistipitata, which comprises the steps of: removing impurities of dry gracilaria tenuistipitata by blanching, air-drying, crushing, and carrying out degreasing treatment by using ethanol; using HCI solution for leaching; centrifugating to obtain a filtrate through ultrasonic extraction; adding NaOH into the filtrate for neutralization; concentrating a supernatant fluid after the centrifugation; adding the ethanol into the concentrated supernatant fluid; standing the obtained solution; washing a precipitate by using the ethanol after the centrifugal precipitation; obtaining a crude sulfated polysaccharide product A by decompression drying; preparing the crude polysaccharide A into aqueous solution; adding the ethanol into the aqueous solution; precipitating to obtain algin B; centrifugating the algin B; adding the ethanol into a supernatant fluid; centrifugating and precipitating the supernatant fluid; using the ethanol and acetone for immersion cleaning; and drying to obtain sulfated polysaccharide C. The method combines the advantages of hot water, ultrasonic wave and acid methods and adopts an ultrasonic wave-assisted thermal acid method to extract the sulfated polysaccharide from the gracilaria tenuistipitata, has larger extraction amount of the sulfated polysaccharide, has the advantages of simple and practical process, low needed investment during the production and quick response, and has the technology which is suitable to be widely popularized.

Description

technical field [0001] The invention belongs to the field of chemical food, in particular to a method for ultrasonic acid extraction of gramicola polysaccharide sulfate. Background technique [0002] Polysaccharides are widely found in higher plants, algae, bacteria and animals in nature, and are important components of living matter. It not only controls cell division, but also plays an important role in regulating cell growth and maintaining the normal metabolism of living organisms. Since the 1960s, research on polysaccharides has attracted more and more attention and attention from researchers. [0003] The ocean occupies one-third of the total area of ​​the earth, contains extremely rich biological resources, and is the largest producer of organic matter. Seaweed is an important part of marine biological resources, the original producer of organic matter in the ocean and the natural enricher of inorganic matter (including chlorine, bromine, iodine and other halogens)....

AI Technical Summary

Problems solved by technology

Domestic research on Gracilaria mainly focuses on the physical and chemical properties of various Gracilaria agars and their effects on seasonal changes and different generations, the classification of agars, the composition of each fraction and their chemical structures, etc. There are few studies on the extraction and preparation of esters, and they focus on methods such as hot water and ultrasonic waves. However, extraction with hot acid solution makes it difficult to dissolve alginic acid at a lower pH, and can also increase the extraction rate of polysaccharide sulfate esters.

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