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Method for producing microbial fermentation bait for sea cucumbers

A technology of microbial fermentation and production method, applied in the field of microbial fermentation bait for sea cucumber, can solve the problems of immature and non-standard production technology, poor quality of sea mud and sea floating mud, unstable use effect, etc., and achieves easy popularization and production cycle. Short, easy-to-manipulate effects

Inactive Publication Date: 2009-12-30
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1. The quality of the raw materials taken is not good, and the use effect is unstable
2. The nutrient composition in the formula is unreasonable, and some nutrient elements are lacking
3. The production process is immature and not standardized
4. The quality of sea mud and sea floating mud used is poor, the content of microorganisms is low, and the effect of the prepared bait is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. The liquid seed fermentation steps of Saccharomyces cerevisiae and Candida (producing protease, amylase, purifying water, etc.) are as follows:

[0033] (1) Seed parent activation: respectively transplant the seed stocks of Saccharomyces cerevisiae and Candida into newly prepared slant medium at a temperature of 28° C. and cultivate for 72 hours;

[0034] The slant medium used for seed activation culture consists of: 20wt% potato (potato) extract 1000mL, sucrose 20g, agar 18g, PH6.8;

[0035](2) Seed fermentation culture (selection medium and fermentation condition test): take the submerged fermentation method to produce liquid seeds, and connect the respectively activated Saccharomyces cerevisiae and Candida seed mothers (Kerschia flasks) into the seed fermentation medium Fermentation, the fermenter can be a 50-liter tank (or a 100-liter, 500-liter, or 1-ton tank), with a liquid volume of no more than 70%, aerated fermentation culture, a culture temperature of 28°C,...

Embodiment 2

[0051] Saccharomyces cerevisiae, Candida, Bacillus subtilis, lactic acid bacteria, and photosynthetic bacteria are used as beneficial microbial flora, and the beneficial microbial flora mixed fermentation seed liquid solid fermentation operation steps are as follows:

[0052] (1) Using soybean meal, bran, rice bran, soy sauce residue, sea rainbow meat, seaweed, and sea floating mud as fermentation raw materials, the weight ratios of various raw materials in the fermentation raw materials are: 10 parts of soybean meal, 10 parts of bran, 10 parts of rice bran, 20 parts of soy sauce residue, 10 parts of sea rainbow, 10 parts of seaweed, 30 parts of sea floating mud; mix the raw materials according to the above ratio, crush and mix evenly according to the basic requirements of the routine, and then place it at a temperature of 25-30 ℃ Reserve in a sterile clean room;

[0053] (2) At room temperature, mix the suspension of high-quality microbial flora (Saccharomyces cerevisiae, Can...

Embodiment 3

[0061] The fermented sea cucumber bait fermented with microbes was tested in juvenile ginseng. The effect of bait fermented with microbes on sea cucumber weight gain is shown in Table 2.

[0062] Table 2. Effects of microbial fermented bait for sea cucumbers on weight gain of sea cucumbers

[0063] Test treatment

pool number

Water body

(m)

Weight before test

(g / pool)

Number of sea cucumbers

(tail / kg)

Feeding amount

(g dry material)

post-test reference

Heavy

(g / pool)*

weight gain

(%)

Woven bag fermentation material 1

Woven bag fermentation material 2

average

8

7

14.5

14.5

6400

8000

760

210

384

480

7900

9800

23.4

22.5

22.9

Plastic bucket fermentation material 1

Plastic bucket fermentation material 2

average

3

2

15.1

...

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Abstract

The invention relates to microbial fermentation bait, in particular to a method for producing microbial fermentation bait for sea cucumbers. Soya bean meal, bran, rice bran, soy sauce residue, mussels, algae and sea floating sludge taken as fermentation raw materials, and Saccharomyces cerevisiae, Candida, Bacillus subtillis, lactobacillus and photosynthetic bacteria taken as beneficial microflora are subjected to inoculation at a temperature of between 25 and 30 DEG C for solid fermentation to produce the microbial fermentation bait for sea cucumbers. The method has the characteristics of simple production process, easy operation, wide sources of production raw materials, low cost, easy promotion, short production period, more fermentation products, good product quality, easy application and the like. When applied in sea cucumber culture, the microbial fermentation bait can improve the survival rate and immunity of the sea cucumbers, quicken the growth and development of the sea cucumbers, improve the ecological environment of culture pond water and purify water quality, and is ideal good-quality microbial fermentation bait for the sea cucumbers.

Description

technical field [0001] The invention relates to microbial fermented bait, in particular to a production method of microbial fermented bait for sea cucumbers. Background technique [0002] In recent years, with the success of sea cucumber artificial breeding technology and the improvement of sea cucumber breeding level, as well as the rising prices of finished ginseng in domestic and foreign markets, the sea cucumber aquaculture industry has developed rapidly, and the sea cucumber bait industry has also developed rapidly. One of the important supporting industries. At present, the demand for sea cucumber bait is large, but raw materials are scarce, the quality is poor, and the market is chaotic. Sea cucumber bait has become one of the limiting factors for sea cucumber breeding and breeding production. Microbial fermentation bait that can promote the rapid growth of sea cucumber is very necessary. Therefore, the research on sea cucumber bait has been paid more and more attent...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/00A23K10/12A23K50/80
CPCY02A40/818
Inventor 薛德林胡江春王国安张仲良刘丽王书锦
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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