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Kit for genetic detection of obesity

A kit, technology for obesity, applied in the field of molecular biology and medicine, can address the problems of increased risk of obesity, promotion of fat, etc.

Inactive Publication Date: 2009-12-23
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When carrying the risk genotype, the HindIII enzyme cleavage site appears on the gene, which will promote the centripetal accumulation of fat and increase the risk of obesity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Use of detection kits

[0029] Step 1: Extraction of DNA template

[0030] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0031] Step 2: Real-time quantitative PCR reaction

[0032] Use the fluorescent quantitative PCR kit in the detection kit to perform 4 independent fluorescent quantitative PCR reactions. The total volume of each reaction is 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10 X fluorescent quantitative PCR reaction buffer, 0.1μl 25mM dNTP mix, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antisense primer, 0.25 μl each of 10 μM fluorescent probe with VIC and fluorescent probe with FAM, and 5.325 μl of deionized water.

[0033] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 50° C. for 2 minutes, 95° C. for 10 minutes, and 60 cycles of 95° C. for 30 seconds and 60° ...

Embodiment 2

[0037] Example 2. Application of detection kits for individual obesity genetic susceptibility testing services

[0038] Step 1: DNA Extraction

[0039] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0040] Step 2: Genotyping Assays

[0041] Use the kit provided by the present invention to carry out fluorescent quantitative PCR on the XbaI SNP site on the APOB gene of the subject's genomic DNA, the G132A SNP site on the LEP gene, the HindIII SNP site on the LPL gene and the Ala55Val SNP site on the UCP2 gene Detect and determine the genotypes of these 4 SNP sites.

[0042] Step 3: Analysis of Individual Genetic Susceptibility to Obesity

[0043] Through the analysis of the SNP genotype of the tested subjects, an analysis report sheet of the individual genetic susceptibility to obesity is issued. The ...

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PUM

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Abstract

The invention discloses a kit for genetic detection of diabetes. The kit comprises a specific primer pair and a specific probe pair which are used for detecting the mononucleotide polymorphism loci genotype of an apolipoprotein B gene (APOB), a leptin gene (LEP), a lipoprotein lipase gene (LPL) and an uncoupling protein 2 gene (UCP2), a fluorescent quantitative PCR general component and the like. The kit can evaluate the genetic risk of individuals suffered from the obesity by detecting the polymorphism loci genotype of the genes closely related to the genetic risk of the obesity at the same time.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, more specifically, the invention relates to a kit for detecting the genetic susceptibility of individual obesity, through simultaneous detection of the apolipoprotein B gene (APOB) and leptin Gene (LEP), lipoprotein lipase gene (LPL), uncoupling protein 2 gene (UCP2) single nucleotide polymorphism loci genotype to assess individual genetic susceptibility to obesity. Background technique [0002] Obesity refers to excessive accumulation and / or abnormal distribution of body fat and weight gain, and is a multifactorial chronic metabolic disease. Genetic factors, high-calorie, high-fat diet, and low physical activity are the main causes of obesity. The World Health Organization (WHO) has defined obesity as a disease, which has a very close relationship with diseases such as hypertension, hyperlipidemia, type 2 diabetes, and atherosclerosis. Obesity leads to the prevalence and The deat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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