Genetic information and health evaluating management
A technology of health assessment and genetic risk, applied in the fields of molecular biology and medicine, can solve the problems of inability to determine the real cause of cardiovascular disease, hysteresis, complex mechanism of cardiovascular disease, etc.
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Embodiment 1
[0038] Example 1. Use of detection kits
[0039] Step 1: Extraction of DNA template
[0040] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.
[0041] Step 2: Electrophoretic detection and typing
[0042] Use the electrophoresis detection set in the detection kit, which contains the following primer pairs:
[0043] Sense primer: 5'-CTGGAGACCACTCCCCATCCTTTTCT-3' (SEQ ID NO: 1)
[0044] Antisense primer: 5'-GATGTGGCCATCACATTCGTCAGAT-3' (SEQ ID NO: 2)
[0045] The reaction system has a total volume of 25 μl, including 1 μl of DNA template with a concentration of 12.5ng / μl, 2.5 μl of 10XPCR reaction buffer, 0.2 μl of 25mM dNTP mixture, 1.5 μl of 25mM MgCl2 solution, 0.125 μl of 5units / μl Taq DNA polymerase, 20 μM ACE gene electrophoresis detection primer pair 0.25 μl each, deionized water 19.175 μl.
[0046] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 94° C. for 12 minutes, and 30 cycles of 94° C...
Embodiment 2
[0071] Example 2. Services for Individual Cardiovascular Health Assessment and Management of People
[0072] Step 1: DNA Extraction
[0073] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.
[0074] Step 2: Genotyping Assays
[0075] Using the kit provided by the present invention, carry out PCR amplification and electrophoresis detection on the SNP site No. rs4646994 on the ACE gene of the subject's genomic DNA, and perform SNP site No. rs5186 on the AGTR1 gene, SNP site No. rs5443 on the GNB3 gene, The SNP site rs1799983 on the NOS3 gene was detected by fluorescent quantitative PCR to determine the genotypes of the four SNP sites.
[0076] Step 3: Analysis of Individual Genetic Risk of Cardiovascular Disease
[0077] Through the analysis of the SNP genotype of the tested subjects, an analysis report ...
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