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High-efficiency extraction method of effective ingredients in golden camellia tea and application thereof in cigarette preparation

An extraction method and technology of golden flower tea, applied to the efficient extraction of effective components in golden flower tea and its application in the preparation of cigarettes, can solve problems such as applications that have not yet been reported, achieve broad market prospects, accelerate intensive processing, The effect of improving the overall quality

Active Publication Date: 2009-12-23
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on the research of Jinhua tea mainly focus on the extraction of its water-soluble components, or the extraction of alcohol-soluble components, and the theoretical research on its biological activity, while its application is mainly tea drinks or health drinks, and other aspects applications of which have not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Extract the active ingredients of golden camellia tea

[0022] 1. Ultra-fine crushing: Use an ultra-fine mechanical grinder to crush the golden flower tea leaves to reach 500 mesh;

[0023] 2. Biotechnology enzymatic hydrolysis:

[0024] Cellulase enzymatic hydrolysis: first add water 5 times the mass of golden camellia tea powder to the golden camellia tea powder, add cellulase at a mass ratio of 1‰, pH4, temperature 30°C, time 5hr, stirring speed 30rpm;

[0025] Protease enzymatic hydrolysis: neutral protease, acid protease, trypsin, papain, bromelain are mixed in equal proportions, then the protease mixture is 1‰ by mass, and the pH of the reaction system is adjusted to 7 with phosphoric acid and potassium hydroxide. 70°C, time 1hr, stirring 10rpm;

[0026] 3. Stirring and alcohol extraction: add 95% ethanol with 5 times the mass of golden camellia tea powder, temperature 50°C, stirring speed 30rpm, seal, reflux extraction time 5hr;

[0027] 4. Separation by ho...

Embodiment 2

[0032] 1. Extract the active ingredients of golden camellia tea

[0033] 1. Ultra-fine crushing: use the ultra-fine mechanical grinder to crush the golden flower tea leaves to reach 2500 mesh;

[0034] 2. Biotechnology enzymatic hydrolysis:

[0035] Cellulase enzymatic hydrolysis: first add water 5 times the mass of golden camellia tea powder to the golden camellia tea powder, add cellulase at a mass ratio of 10‰, pH 7, temperature 70°C, time 1hr, stirring speed 10rpm;

[0036] Protease enzymatic hydrolysis: The composite protease Protamex was used in a mass ratio of 10‰, and the pH value of the reaction system was adjusted to 4 with phosphoric acid and potassium hydroxide, the temperature was 30°C, the time was 5hr, and the stirring speed was 30rpm;

[0037] 3. Stirring alcohol extraction: add 95% ethanol with 20 times the mass of golden camellia tea powder, temperature 80°C, stirring speed 5rpm, seal, reflux extraction time 0.5hr;

[0038] 4. Separation by horizontal filte...

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Abstract

The invention discloses a high-efficiency extraction method of effective ingredients in golden camellia tea and the application thereof in cigarette preparation. The extraction method comprises the steps of crushing, enzymolysis, alcohol extraction, separation and concentration, wherein the enzymolysis is carried out by using cellulose and prolease. The application of the extraction method in cigarette preparation comprises the following steps: adding extracted effective-ingredient concentrated solution of golden camellia tea into a cigarette group according to a mass ratio of 0.1 per thousand to 10 per thousand to prepare cigarettes. By adopting the extraction method, the effective ingredients in the golden camellia tea are extracted with high efficiency and low cost. The extracted effective-ingredient concentrated solution of the golden camellia tea is applied to the cigarette preparation and changes smoke gas to be soft, delicate and clear, the irritation is reduced, residues reduced in the oral cavity are reduced, the sweet backing sense is obviously strengthened, and the cigarette quality is obviously improved.

Description

technical field [0001] The invention relates to a high-efficiency extraction method of effective components in golden camellia tea leaves and its application in cigarette preparation. Background technique [0002] Golden Camellia belongs to the Camellia family Theaceae and is one of the most precious plant varieties in the world. The main production area in my country is Guangxi, and it has become a characteristic resource advantage plant with great development value in Guangxi. Golden camellia tea leaves are rich in nutrients, rich in soluble sugars, proteins, amino acids, trace elements, tea saponins, flavonoids and other ingredients, and have the functions of clearing heat and detoxification, diuresis and dampness, anti-oxidation, and anti-aging. At present, the reports on the research of Jinhua tea mainly focus on the extraction of its water-soluble components, or the extraction of alcohol-soluble components, and the theoretical research on its biological activity, while...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P39/06A61P7/10C11B9/00A24B15/30
Inventor 饶国华
Owner CHINA TOBACCO GUANGDONG IND
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