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Method for extracting water-soluble beta-glucan with high purity from grain bran

A water-soluble and glucan technology, applied in the field of grain deep processing, can solve the problems of less processing, instability, and damage to the structure of water-soluble dextran, and achieve the effect of lowering cholesterol, mild conditions, and simple process

Active Publication Date: 2009-12-16
WENGYUAN GUANGYE QINGYI FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The extracted water-soluble dextran solution has a high viscosity and is not easy to be centrifuged. The extract obtained by one-time purification method contains more impurities, and the purity of the final product is difficult to reach more than 70%;
[0008] (2) The conventional purification method will destroy the structure of water-soluble dextran, reduce the solubility of the product and be unstable, and it is easy to precipitate in aqueous solution, so it is not suitable for application in beverages;
[0009] (3) Purification by column chromatography can be achieved, but the amount of treatment is small and the production cost is high, which is not conducive to industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 100kg of oat bran raw material, add 300kg of water, stir evenly, adjust the pressure to 10MPa, after high-pressure homogenization, raise the temperature of the homogenized solution to 30°C, pH is 7, and continue to stir for 5 hours to promote the dissolution of oat bran cell wall and water-soluble β- For dextran, adjust the temperature to 30°C under normal pressure, add 30g of cellulase, adjust the pH to 9, keep stirring at a constant temperature for 2 hours, use a hanging bag centrifuge, the centrifugal speed is 1000rpm, the filter bag is made of polyester material, 400 pore size, centrifuged to collect the supernatant, added yeast 100g, fermented at 28°C for 24h, heated to 60°C, kept at a constant temperature for 15min, flocculated and precipitated, centrifuged with a high-speed centrifuge at a speed of 10,000r / min, and centrifuged for 10min, the obtained The fermented liquid is spray-dried to obtain water-soluble β-glucan. The appearance of the obtained product i...

Embodiment 2

[0033] Take 100kg of oat bran raw material, add 500kg of water, stir evenly, adjust the pressure to 20MPa, after high-pressure homogenization, raise the temperature of the homogenized solution to 50°C, pH is 7, and continue to stir for 3 hours to promote the dissolution of oat bran cell wall and water-soluble β- For dextran, cool down to 40°C under normal pressure, add 150g of cellulase, adjust the pH to 6, keep stirring at a constant temperature for 2.5h, use a hanging bag centrifuge, the centrifugal speed is 1000rpm, the filter bag is made of polyester material, 400 pore size, centrifuged to collect the supernatant, added 200g of yeast, fermented at 30°C for 2h, heated to 80°C, kept at a constant temperature for 20min, flocculated and precipitated, centrifuged in a high-speed centrifuge at a speed of 10,000r / min, and centrifuged for 10min, the obtained The fermented liquid is spray-dried to obtain water-soluble β-glucan. The appearance of the obtained product is a very fine w...

Embodiment 3

[0035] Take 100kg of barley bran raw material, add 1200kg of water, stir evenly, adjust the pressure to 15MPa, after high-pressure homogenization, raise the temperature of the homogenized solution to 70°C, pH is 7, and continue to stir for 2 hours to promote the dissolution of water-soluble β- For dextran, cool down to 43°C under normal pressure, add 200g cellulase, adjust the pH to 6, keep stirring at constant temperature for 1 hour, use a hanging bag centrifuge, the centrifugal speed is 1000rpm, the filter bag is made of polyester material, 400 mesh pore size, centrifuged to collect the supernatant, add yeast 180g, ferment at 30°C for 2 hours, heat to 70°C, keep the temperature for 10 minutes, flocculate and precipitate, and use a high-speed centrifuge to centrifuge at a speed of 10,000r / min for 10 minutes. The product is spray-dried to obtain water-soluble β-glucan. The appearance of the obtained product is a very fine white powder. The product purity reaches more than 70% a...

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PUM

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Abstract

The invention discloses a method for extracting water-soluble beta-glucan with high purity from grain bran, comprising the following steps: taking grain bran as raw material, adding water and mixing evenly, carrying out high-pressure homogeneity, extraction, enzymolysis to obtain primary purifying solution, then adding yeast for fermentation, heating and precipitating protein and centrifuging to obtain two-stage purifying solution, spraying and drying to obtain the water-soluble beta-glucan product with high purity. The extraction method has the advantages of simple process, easy operation and low cost, and the prepared water-soluble beta-glucan has high purity and activity and fine and stable solubility, which is applicable to various drinks.

Description

technical field [0001] The invention belongs to the technical field of grain deep processing, and in particular relates to a method for extracting high-purity water-soluble β-glucan from grain bran. Background technique [0002] Water-soluble β-glucan is a polymer hydrocolloid connected by β-1,3 and β-1,4 glycosidic bonds, the ratio of the two glycosidic bonds is roughly 3:7, mainly found in oats, barley The cell wall endosperm layer of grains such as barley, barley, wheat, etc. Its content varies greatly with the variety, place of origin, and processing methods. Different processing and milling will greatly affect the water-soluble β-glucose in the grain bran. The content of polysaccharides generally ranges from 1% to 15%, and the distribution of molecular weight is 5×10 5 and 3×10 6 According to Dalton, water-soluble β-glucan not only serves as a functional factor to reduce low-density lipoprotein in human body, but also has potential food application value, which can be...

Claims

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Application Information

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IPC IPC(8): C08B37/02C12S3/02
Inventor 钟细娥李春荣高展炬詹耀才
Owner WENGYUAN GUANGYE QINGYI FOOD TECH
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