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Preparation method of collagen-hyaluronic acid composite products

A technology of hyaluronic acid and composite materials, applied in medical science, prosthesis, etc.

Inactive Publication Date: 2013-02-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Hyaluronic acid exists in the form of acid in the tissue, and the existing hyaluronic acid extraction, due to the limitation of the process, can only obtain the sodium salt or zinc salt of hyaluronic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation concentration is the sodium hyaluronate solution of 6 grams / liter, puts this solution into the dialysis bag that molecular weight cut off is 3000, in American electrophoresis instrument (Bio-Rad company, GT series horizontal electrophoresis tank, POWER PAC power supply) 15V The electrolytic treatment was performed for 10 hours, and the electrolyte was 0.01M sulfuric acid. Mix 100 ml of the above-mentioned hyaluronic acid obtained through desalting treatment with 100 ml of 6 g / L collagen solution, add 10 g of azocarbodiimide during the compounding process, mix well, pour into the mold, and crosslink for 4 hours at room temperature , the solution was freeze-dried for 24 hours to obtain a homogeneous composite hyaluronic acid-collagen porous material. Then, the porous material product was cross-linked twice at room temperature with a carbodiimide aqueous solution with a mass concentration of 5% for 4 hours, and then freeze-dried for 24 hours to obtain a collage...

Embodiment 2

[0030] Preparation concentration is the sodium hyaluronate solution of 20 grams / liter, puts this solution into the dialysis bag that molecular weight cut off is 3000, in electrophoresis instrument (Bio-Rad company, GT series horizontal electrophoresis tank, POWER PAC power supply) in 25V The electrolytic treatment was performed for 12 hours, and the electrolyte was 0.01M sulfuric acid. Mix 50 ml of the above-mentioned hyaluronic acid obtained through desalination treatment with 50 ml of 4 g / L collagen solution, add 1 g of azocarbodiimide during the compounding process, then add IGF growth factor sustained-release microspheres, and cross-link at room temperature After half an hour, the solution was freeze-dried for 24 hours to obtain a uniformly composited hyaluronic acid-collagen porous material. Then, the porous material product was cross-linked twice at room temperature for 4 hours with a glutaraldehyde aqueous solution with a mass concentration of 5%, and then freeze-dried ...

Embodiment 3

[0032] Prepare the sodium hyaluronate solution with a concentration of 2 g / L, put the solution into a dialysis bag with a molecular weight cut-off of 6000, and place it in a 55V electrophoresis instrument (Bio-Rad company, GT series horizontal electrophoresis tank, POWER PAC power supply) The electrolytic treatment was performed for 3 hours, and the electrolyte was 0.001M sulfuric acid. Mix 100 ml of the above-mentioned hyaluronic acid solution obtained through desalting treatment with 50 ml of 10 g / L collagen solution, add 8 g of azocarbodiimide during the compounding process, and then add bone morphoprotein, mix well, and place at room temperature Combined for 4 hours, and then freeze-dried for 24 hours to obtain a collagen-hyaluronic acid repair material product.

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Abstract

The invention relates to a preparation method of collagen-hyaluronic acid composite products; in the method, electrochemical treatment is first carried out on hyaluronate to remove metal ions in the hyaluronate and obtain hyaluronic acid solution; then the obtained hyaluronic acid solution is mixed with collagen solution to obtain the mixed solution of the collagen and hyaluronic acid; the mixed solution can be applied directly or used for preparing collagen-hyaluronic acid composite products by the method of freeze drying.As the method of the invention does not affect the biological properties of natural biological materials, the final products still retain good biological activity.

Description

technical field [0001] The invention relates to the technical field of collagen-hyaluronic acid composite materials, in particular to a preparation method of collagen-hyaluronic acid composite material products. Background technique [0002] With the development of modern society, there are more and more tissue damage problems caused by various reasons. Such as skin injuries caused by burns, scalds, knife wounds, firearm injuries, etc., limb blood vessel and nerve injuries caused by traffic accidents, loss of surrounding tissues caused by tumor resection, and articular cartilage and meniscus damage caused by sports injuries and pathological injuries. damage etc. There are various reasons for the injury, and the final result is the damage of connective tissues such as articular cartilage, meniscus, skin, blood vessels and nerves. Small-area, small-scale, low-injury tissue damage can be repaired automatically by the human body through the mobilization of autologous tissues a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/40A61L27/54A61L27/56A61L27/24A61L27/20
Inventor 吴刚王迎军
Owner SOUTH CHINA UNIV OF TECH
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