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Method for obtaining transformable substrate by using fungi leftovers

A technology for bacterial bran and substrate, which is applied in the field of obtaining convertible substrates, can solve the problems of low added value of the overall utilization of bacteria bran, and achieve the effects of improving resource value and reducing energy consumption and cost.

Inactive Publication Date: 2009-12-02
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The output of edible and medicinal fungus chaff is huge in my country. At present, there are precedents in the research of using fungus chaff to produce feed, single-cell protein, and extraction of SOD. However, the overall utilization of fungal chaff and the added value of the obtained products are relatively low, and According to the characteristics of lignocellulose in fungal chaff, it has not been reported that it is used in the production of high value-added convertible substrates.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 utilizes Pleurotus ostreatus fungus chaff to obtain transformable substrate preparation technology

[0020] Air-dry the chaff of Pleurotus ostreatus and crush it for alkali treatment. The condition of alkali treatment is: adopt 0.04mol / L NaOH solution, the solid-liquid ratio of bacteria chaff and lye is 1:50, H 2 o 2 The concentration is 1%, the alkali treatment temperature is 30°C, and the alkali treatment time is 24h. After alkali treatment, the cellulose content increased significantly, from 35.5% to 46.6%, and the lignin content decreased significantly, from 24.1% to 11.6%, and the lignocellulose network structure was severely damaged. Alkali treatment caused a mass loss of 27.9% of the fungus chaff, and the main substances lost were lignin and soluble substances. The alkali-treated fungus chaff sample was neutralized with dilute sulfuric acid and then centrifuged, and the filter residue was dried at 100°C and used for cellulase enzymatic hydrolysis ...

Embodiment 2

[0022] Embodiment 2 utilizes Pleurotus eryngii mushroom chaff to obtain transformable substrate preparation process

[0023] Pleurotus eryngii fungus chaff is air-dried and crushed for alkali treatment. The conditions of alkali treatment are: using 0.1mol / L NaOH solution, the solid-liquid ratio of bacterial chaff and alkali solution is 1:20, H 2 o 2 The concentration is 2%, the alkali treatment temperature is 100°C, and the alkali treatment time is 0.75h. After alkali treatment, the cellulose content increased significantly, from 33.5% to 54.9%; hemicellulose was also concentrated, and the content increased from 22.7% to 36.2%; lignin was severely damaged, and the content decreased significantly, from 14.3% to 4.8% %. The alkali-treated fungus chaff sample was washed with tap water until neutral, and the filter residue was dried at 100°C for cellulase enzymatic hydrolysis and saccharification. The cellulase used is fermented by Trichoderma reesei, and the saccharification ...

Embodiment 3

[0025] Embodiment 3 utilizes Flammulina velutipes mushroom chaff to obtain transformable substrate preparation process

[0026] The chaff of Flammulina velutipes is air-dried and pulverized for alkali treatment. The alkali treatment conditions are as follows: 0.2mol / L NaOH solution is used, the solid-to-liquid ratio of bacterial chaff and alkali solution is 1:20, the alkali treatment temperature is 100°C, and the alkali treatment time is 0.5h. After alkali treatment, the cellulose content increased significantly, from 31.5% to 53.2%, and the lignin content decreased significantly, from 24.1% to 5.6%. Alkali-treated fungus chaff samples were washed with tap water until neutral, and then directly used for cellulase enzymatic hydrolysis and saccharification. The cellulase used is fermented by Trichoderma reesei, and the conditions of saccharification are as follows: the dosage of cellulase is 10 IU / g bacterial chaff, the solid-liquid ratio is 1:50, the enzymolysis temperature is...

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Abstract

The invention provides a method for obtaining a transformable substrate by using fungi leftovers, which comprises two steps of diluted alkaline pretreatment and cellulase saccharification. The method uses the fungi leftovers after fruiting as raw materials, changes waste materials generated by edible fungi and medicinal fungi into valuable, and not only improves the economic benefit of the productions of the edible fungi and the medicinal fungi, but also finds a new raw material for biorefinery. The method has the advantages of simple flow, simple reagent, low cost, and suitability for popularization and application.

Description

technical field [0001] The invention relates to a technology for obtaining a transformable substrate, in particular to a method for obtaining a transformable substrate by using the chaff of edible and medicinal fungi (the leftovers left after the cultivation of edible and medicinal fungi). Background technique [0002] Mushroom chaff is the leftovers left after the cultivation of edible and medicinal fungi, and its main raw material is the straw of crops. After the edible and medicinal fungi are cultivated with crop stalks, the utilization rate of cellulose therein is very low, generally only 20-30%. Most edible and medicinal fungi belong to white rot fungi or brown rot fungi, which can destroy lignocellulose network structure, degrade lignin and depolymerize cellulose. Therefore, after cultivation of edible and medicinal fungi with crop stalks, more cellulose remains. [0003] The output of edible and medicinal mushroom chaff is huge in my country. At present, there are p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 张晓昱余洪波徐春燕邬建国
Owner HUAZHONG UNIV OF SCI & TECH
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