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Ophiopogon japonicus extract, preparation method and hypoglycemic application thereof

A technology of Ophiopogon japonicus extract and Ophiopogon japonicus, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of affecting the quality of preparations, cumbersome steps, high cost, etc., to improve intake capacity, protect islet cells, and enhance absorption capacity Effect

Active Publication Date: 2009-10-21
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many chemical reactions involved in the process, the steps are cumbersome, and there are problems such as long cycle, high cost, low yield, poor stability, etc., which seriously affect the quality of the preparation

Method used

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  • Ophiopogon japonicus extract, preparation method and hypoglycemic application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 200 grams of Ophiopogon japonicus herb, add water to decoct and extract once, add 20 times the amount of water, decoct for 2 hours, concentrate the decoction until the density is 1.05g / ml, add ethanol until the alcohol content is 80%, let stand overnight, and precipitate After filtration and drying, a light yellow crude product was obtained. The crude product was dissolved in water, purified with dextran Sephadex G-75 column, and eluted with water; the eluate was detected by sulfuric acid-anthrone method, and the positive reaction part was collected and freeze-dried to obtain white loose flocculent crystals, namely wheat The winter extract has a sugar content of 90% (calculated as fructose) after analysis, and a molecular weight range of 500-2000.

Embodiment 2

[0019] Take 200 grams of Ophiopogon japonicus herb, add water to decoct and extract once, add 10 times the amount of water, decoct for 2 hours, decoct the extract to a density of 1.18g / ml, add ethanol until the alcohol content is 50%, and let it stand overnight , the precipitate was filtered and dried to obtain a light yellow crude product. The crude product was dissolved in water, purified with dextran Sephadex G-50 column, and eluted with water; the eluate was detected by sulfuric acid-anthrone method, and the positive reaction part was collected and freeze-dried to obtain white loose flocculent crystals, namely wheat The winter extract has a sugar content of 96% (calculated as fructose) after analysis, and a molecular weight range of 500-2000.

Embodiment 3

[0021] Take 200 grams of Ophiopogon japonicus herb, decoct and extract twice with water, add 5 times the amount of water, decoct for 2 hours, combine the decoction extracts twice, concentrate to a density of 1.08g / ml, add ethanol until the alcohol content is 60 %, left overnight, the precipitate was filtered and dried to obtain a light yellow crude product. The crude product was dissolved in water, purified with dextran Sephadex G-35 column, and eluted with water; the eluate was detected by sulfuric acid-anthrone method, and the positive reaction part was collected and freeze-dried to obtain white loose flocculent crystals, namely wheat The winter extract has a sugar content of 94% (calculated as fructose) after analysis, and a molecular weight range of 500-2000.

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Abstract

The invention relates to an ophiopogon japonicus extract, a preparation method and the hypoglycemic application thereof. The preparation method of the ophiopogon japonicus extract comprises: ophiopogon medicine is taken to be decocted for 1-3 times, added with water by 5-20 times every time, then decocted for 30-120min every time, and filtered and merged into water-decocted liquid; after being concentrated to the density of 1.05-1.35g / ml, the obtained water-decocted liquid is added with ethanol until the alcohol content has the volume percent of 50-80%, and is collected and precipitated after staying over night; the precipitation is separated and purified by sephadex column, and then processed by vacuum freeze drying, so that white loose flocculent crystal which is the ophiopogon japonicus extract can be obtained, and the extract has the sugar content of 90-98%( based on fructose) as well as the molecular weight within the range of 500-2000. Animal experiments prove that the ophiopogon japonicus extract has remarkable hyperglycemic effect, and the action mechanism of the ophiopogon japonicus extract can be further explained by vitro experiments; the result shows the ophiopogon japonicus extract can reduce blood sugar levels by inhibiting the activity of alpha-glucosaccharase in alimentary canal, reduce the absorption for glucose by intestinal villi and the protective action for islet cells. Therefore, the extract can be used for developing medicine for treating type II diabetes.

Description

technical field [0001] The invention relates to the technology of extracting Ophiopogon japonicus and its application in the preparation of medicines for treating diabetes. Background technique [0002] Ophiopogon japonicus (Thunb.) Ker-Gawl. is the dry tuber root of Liliaceae Ophiopogon japonicus (Thunb.) Ker-Gawl. Pharmacological and clinical trials have shown that the crude polysaccharide extracted from Ophiopogon japonicus can effectively reduce the blood sugar level of experimental diabetic mice, reduce the fasting blood sugar level and 2h postprandial blood sugar level of patients with type II diabetes, and improve insulin resistance. [0003] In the prior art, the purification of the crude polysaccharides of Ophiopogon japonicus generally uses dried Ophiopogon japonicus as raw material, adopts the method of "water extraction and alcohol precipitation" to obtain its crude extract, then precipitates the protein with chloroform-n-butanol, and finally decolorizes it with ...

Claims

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Application Information

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IPC IPC(8): A61K36/8968A61P3/10
Inventor 苏薇薇丁林伟冯国培许定舟杨翠平王永刚刘碧珊梁秉中
Owner SUN YAT SEN UNIV
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