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Helicobacter pylori viable bacteria carrier vaccine and special recombination bacteria thereof

A technology of Helicobacter pylori and recombinant bacteria, applied in the direction of recombinant DNA technology, the use of vectors to introduce foreign genetic material, bacteria, etc., can solve the problems of less than 20% cure rate, side effects of drugs, poor patient compliance, etc.

Active Publication Date: 2009-09-23
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Problems solved by technology

[0004] At present, antibiotic therapy is mainly used clinically to remove H. pylori, but the cure rate of H. pylori with a single antibiotic in the body is less than 20%. After triple therapy, that is, bismuth plus two antibiotics, the cure rate of H. 55-95%, and there are the following problems: high cost, low eradication rate, continuous increase of drug-resistant strains, side effects of drugs, recurrence and reinfection after drug withdrawal, poor patient compliance, and cannot be used to prevent H .pylori infection and other issues

Method used

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  • Helicobacter pylori viable bacteria carrier vaccine and special recombination bacteria thereof
  • Helicobacter pylori viable bacteria carrier vaccine and special recombination bacteria thereof
  • Helicobacter pylori viable bacteria carrier vaccine and special recombination bacteria thereof

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Embodiment 1

[0034] Embodiment 1, preparation and effect of recombinant Shigella flexneri and Helicobacter pylori live bacteria vector vaccine

[0035] 1. Preparation of recombinant Shigella flexneri and Helicobacter pylori live bacterial vector vaccines

[0036] 1. Cloning of Helicobacter pylori urease B subunit (ureB) gene

[0037] According to the ureB gene sequence of Helicobacter pylori 26695, primers were designed with PCR primer design and analysis software (P1: 5'-TGCGAGCTCAAAAAGATTAGCAGAAAA-3'; P2: 5'-GGCTGCAGGAAAATGCTAAAGAGTTG-3'), and Helicobacter pylori 26695 (purchased from Genomic DNA from the Chinese Center for Disease Control and Prevention) was used as a template, and the high-fidelity Pyrobest DNA polymerase (purchased from Takala Company) was used to carry out PCR amplification to obtain the H.pylori ureB gene, such as figure 1 shown.

[0038] After purification, the ureB gene fragment amplified by PCR was digested with SacI and PstI (purchased from NEB Company) and liga...

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Abstract

The invention relates to a helicobacter pylori viable bacteria carrier vaccine and special recombination bacteria thereof. The special recombination bacteria is recombination Shigella flexneri (Sh.flexneri) which is obtained by introducing coding gene of fusion protein containing helicobacter pylori urease B subunit and helicobacter pylori heat shock protein A subunit into the Shigella flexneri (Sh.flexneri). The active ingredient of the helicobacter pylori viable bacteria carrier vaccine is the recombined Shigella flexneri (Sh.flexneri). Animal test proves that the helicobacter pylori vaccine bacterial strain has favorable immunogenicity and protective immunity.

Description

technical field [0001] The invention relates to a live Helicobacter pylori carrier vaccine and special recombinant bacteria thereof. Background technique [0002] Gastritis and peptic ulcer are common and frequently-occurring diseases in humans, and gastric cancer is also one of the malignant tumors with relatively high morbidity and mortality. For many years, people have always believed that this kind of disease is a common medical disease, and has no connection with the infection of pathogens. The medical profession once believed that in the acidic environment of the stomach, it is difficult for microorganisms to settle and multiply to cause disease. Gastric acid is a key factor in the formation of ulcers, and neutralizing gastric acid or inhibiting gastric acid secretion is usually used clinically to treat such diseases. However, in 1982, two Australian scholars, Robin Warren and Barry Marshall, isolated Helicobacter pylori (H. pylori) from human gastric tissue. Afterwa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21A61K39/02A61P31/04A61P1/04C07K19/00C12N15/62C12N1/00C12N15/63C12R1/01
CPCY02A50/30
Inventor 刘纯杰张兆山陶好霞王令春袁盛凌展德文王芃王艳春
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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