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Anthropogenic H5N1-resisting hemagglutinin protein broad-spectrum neutralising antibody and application thereof

A technology of hemagglutinin protein and antibody, applied in the direction of antiviral immunoglobulin, application, antibody, etc., can solve the problems of infection, limited application, large-scale preparation limitation, etc., and achieve the effect of strong penetrating power

Inactive Publication Date: 2009-09-23
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most McAbs are of mouse origin, and the heterogeneous reaction of mouse McAbs greatly limits the application of McAbs as therapeutic agents in humans.
As an antibody component, immunoglobulin (Vaccinia immune globulin, VIG) mainly comes from the immune serum of donors (convalescent patients), and it takes a lot of manpower and financial resources to obtain positive serum and pass the safety test, which makes its mass production difficult. Restricted, and because it comes from serum, it is prone to infection of blood-borne diseases

Method used

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  • Anthropogenic H5N1-resisting hemagglutinin protein broad-spectrum neutralising antibody and application thereof
  • Anthropogenic H5N1-resisting hemagglutinin protein broad-spectrum neutralising antibody and application thereof
  • Anthropogenic H5N1-resisting hemagglutinin protein broad-spectrum neutralising antibody and application thereof

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Embodiment 1

[0035] Materials and methods

[0036] 1. Viruses, cells, vectors

[0037] Human H5N1 isolates A / Anhui / 1 / 2005 (Genebank accession number DQ371928), A / Guangxi / 1 / 2005 (DQ371930), A / Hunan / 1 / 2006 (FJ492879), A / Zhejiang / 1 / 2006 (FJ492880 ), A / Sichuan / 1 / 2006(FJ492881), A / Fujian / 1 / 2005(FJ492882), A / Fujian / 1 / 2007(FJ492883), A / Guangdong / 1 / 2006(FJ492884), A / Jiangxi / 1 / 05(FJ492885), A / Xinjiang / 1 / 2006(FJ492886), A / VietNam / 1203 / 2004(AY818135), A / HongKong / 156 / 1997(AF023709), A / Turkey / 15 / 2006(EF619989) , A / Indonesia / 5 / 2005 (EU146622) provided by the Institute of Virology, Chinese Center for Disease Control and Prevention and the US Centers for Disease Control and Prevention. The bacterial strain is XLI-Blu (Stratagene, USA); the phage expression vector is pComb3 (40kb), donated by Scripps Research Institute, USA; the phage used is VCSM13 (Invitrogene Corporation, USA). Baculovirus expression vector is pAC-L-Fc (German PROGEN PR3003) (Liang, M.F., Stefan, D., Li, D.X., Queitsch, I., Li, W., ...

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Abstract

The invention provides an anthropogenic H5N1-resisting hemagglutinin protein broad-spectrum neutralising antibody. The antibody is obtained by screening through a phage display technology. The sequence of the hypervariable region (CDRs) determining the specificities of the antibody is represented by figure 1. The antibody of the invention specifically identifies H5N1 highly pathogenic avian influenza virus particle antigen, has obvious fluorescence immune reaction and enzyme-coupled immune reaction with the avian influenza virus H5N1 aiming at avian influenza virus hemagglutinin protein HA, and has the neutralising activity function of resisting the avian influenza virus H5N1. The antibody of the invention can be made into specific antibody medicines clinically used for preventing and treating human avian influenza that is caused by the H5N1 avian influenza virus, so as to clinically prevent or treat human avian influenza that is caused by the H5N1 avian influenza virus.

Description

technical field [0001] The invention relates to genetic engineering antibody technology, in particular to a broad-spectrum neutralizing antibody against H5N1 hemagglutinin protein of human origin, and also relates to the application of the antibody in the preparation of medicines for preventing or treating bird flu. Background technique [0002] Highly pathogenic avian influenza (HPAI) is a severe infectious disease of poultry caused by influenza A virus of the Orthomyxoviridae family. The highly pathogenic avian influenza virus H5N1 has been highly concerned by the international community since 1997 when 18 people were infected and 6 died. Especially since 2003, the highly pathogenic avian influenza virus H5N1 animal epidemic has become endemic in Asia. Epidemic, and has spread to Europe and Africa, human infection cases and the countries and regions where human infection cases occur continue to expand. The highly pathogenic avian influenza virus H5N1 has broken through th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13C12N15/63C12N5/10A61K39/42A61P31/16G01N33/577
Inventor 梁米芳孙丽娜李德新舒跃龙刘琴芝李川张全福
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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