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Large-scale industrialized technology for beer yeast extract

A technology of yeast extract and beer waste yeast, applied in animal husbandry, animal feed, animal feed, etc., can solve the problems of low comprehensive utilization rate, low quality, and single product

Active Publication Date: 2009-09-23
SHANDONG BIO SUNKEEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, their shortcomings are: first, the quality of the product is not high compared with foreign products; second, the product is single, and the thick slurry after extracting nucleotides or preparing yeast powder is waste water, which is discharged as waste liquid or has a low comprehensive utilization rate; Its three large-scale industrialization technology is not complete, and there is no production technology practice of 3000t / a beer yeast extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Washing of spent beer yeast: add 1000g of water and 18g of NaHCO to the reactor 3 , stir for 5 minutes, add 500g of waste yeast, stir in water at 10°C for 5 minutes, put the yeast sludge into the drier to remove the slag and set aside

[0015] 2. Debittering: The amount of debittering agent is 1%, and the time is 60 minutes, and 8% water-washed yeast milk is obtained after debittering

[0016] 3. Wall breaking: Add 500 g of 8% yeast sludge into the reactor, and homogenize twice at 60 MPa with a high-pressure homogenizer to break the cell wall.

[0017] 4. Self-containment: After adding 200g of homogeneous broken yeast sludge to 2 times the volume of sterile water and 5% NaCl, stir evenly, adjust the pH to 8 with 4mol / L NaOH, and then add nucleic acid with a mass fraction of 1%. Enzyme, autolyzed at 55°C for 24 hours

[0018] 5. Enzymatic hydrolysis and sterilization: The yeast sludge after self-compatibilization is hydrolyzed with weak acid protease 1# at pH 6.4 an...

Embodiment 2

[0023] 1. Washing of spent beer yeast: add 1000g of water and 18g of NaHCO to the reactor 3 , stirred for 5 minutes, added 500g of spent yeast, stirred in water at 10°C for 5 minutes, put it in a spin dryer to remove slag, and the yeast sludge was set aside.

[0024] 2. Debittering: The amount of debittering agent is 1%, and the time is 60 minutes, and 8% water-washed yeast milk is obtained after debittering

[0025] 3. Wall breaking: Add 500 g of 8% yeast sludge into the reactor, and homogenize twice at 60 MPa with a high-pressure homogenizer to break the cell wall.

[0026] 4. Self-containment: After adding 200g of homogeneous broken yeast sludge to 2 times the volume of sterile water and 5% NaCl, stir evenly, adjust the pH to 8 with 4mol / L NaOH, and then add nucleic acid with a mass fraction of 1%. Enzyme, autolyzed at 55°C for 24 hours

[0027] 5. Enzymatic hydrolysis and sterilization: The yeast sludge after self-compatibilization is hydrolyzed with weak acid protease 2...

Embodiment 3

[0032] 1. Washing of spent beer yeast: add 1000g of water and 18g of NaHCO to the reactor 3 , stirred for 5 minutes, added 500g of spent yeast, stirred in water at 10°C for 5 minutes, put it in a spin dryer to remove slag, and the yeast sludge was set aside.

[0033] 2. Debittering: The amount of debittering agent is 1%, and the time is 60 minutes, and 8% washed yeast milk is obtained after debittering.

[0034] 3. Wall breaking: 500 g of 8% yeast milk was added to the reactor, and the high-pressure homogenizer was used to homogenize twice at 60 MPa to break the cell wall.

[0035] 4. Autolysis: After adding 200g of homogeneous broken yeast sludge to 2 times the volume of sterile water and 5% NaCl, stir evenly, adjust the pH to 8 with 4mol / L NaOH, and then add nucleic acid with a mass fraction of 1%. Enzyme, autolyzed at 55°C for 24 hours.

[0036] 5. Enzymolysis and sterilization: The yeast sludge after self-compatibilization is hydrolyzed with weak acid protease 3# at pH 6...

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Abstract

The invention discloses new large-scale industrialized technology for producing novel yeast extract series products 3,000 t / a by using beer yeast slurry as an initial raw material, and belongs to the technical field of preparing series biological products from yeast. The production steps comprise preliminary treatment, debitterizing process, controllable hydrolysis process, separation and refining, condensation or spray drying. The technology is characterized in that the waste beer yeast with low cost is used as the raw material, the technology combining a physical wall-breaking method, an automatic dissolution method and an enzymolysis method is provided, the hydrolysis ratio of beer yeast protein reaches 55 percent, and the total yield reaches 60 percent. The invention also discloses a biochemical controllable debitterizing process, which solves the technical bottlenecking problems of more impurities, heavy bitter taste and low yield of the yeast slurry raw material. Three series and more than 20 yeast extract series products are successfully obtained. The comprehensive utilization rate of the yeast slurry is over 90 percent; and the technology is typical environment-friendly, clean and resource-saving technology and has remarkable economic and social benefits.

Description

Technical field: [0001] The invention relates to the technical field of producing novel yeast extract series products by using beer waste yeast, in particular to a comprehensive method for simultaneously producing food-grade and industrial-grade yeast extracts by using beer yeast mud. Background technique: [0002] Before the present invention, the scientific and technological achievements of yeast extract as the key project of the National Ninth Five-Year Plan have been transformed into productivity. Some large-scale yeast extract manufacturers all use bread yeast (Bread yeast) and active dry yeast (active dry yeast) Tail powder is used as raw material to produce food-grade yeast extract for domestic sales and exports, but the common problem is that it consumes a lot of money and energy, and it faces challenges in environmental protection and resources. [0003] In recent years, although some use beer waste yeast (mud) as a raw material to simply extract nucleotides or simp...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/228C12N1/18A23K1/06A23K10/38A23L31/15
CPCY02P60/87
Inventor 吴允山易勇严共鸿吴绮文余晓斌
Owner SHANDONG BIO SUNKEEN
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