Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide

A technology of antimicrobial peptide and serum powder, which is applied in antiviral agents, digestive system, medical raw materials derived from arthropods, etc., can solve the problems of unspecified concentration of active E. Achieve rapid drying and sterilizing ability, shorten drying time, and good induction effect

Active Publication Date: 2012-05-09
BIOPHARM RES & DEV CENT JINAN +1
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above-mentioned technical problems, the patent application with the application number 03128069.2 discloses "a preparation method for extracting polypeptide antibiotics from silkworm bodies", but the disadvantage of this application is that the concentration of active E. Live Escherichia coli is bound to cause pollution, and it is impossible to induce antimicrobial peptides by eating Escherichia coli orally; in addition, the induction condition of the ultrasonic wave is 10-20 kHz for 2-5 hours, and the lowest frequency of the ultrasonic wave is above 20 kHz, 10 kHz He is not ultrasonic, and the power of ultrasonic is not specified. Ultrasonic can only be transmitted in water or other carriers. If the silkworm body is immersed in water for 2 to 5 hours, it will inevitably lead to suffocation and death; the activity of the antimicrobial peptide in the silkworm body before extraction Description, so the preparation method of this patent is not feasible

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide
  • Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide
  • Method for preparing silkworm powder, pupa powder or pupa serum powder containing antibacterial peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Inducing the production of antimicrobial peptides and processing methods for silkworm cooked silkworms, comprising the following steps:

[0044]1. Collection of cooked silkworms: Collect silkworm larvae at the end of the fifth instar of silkworms, remove residual mulberry and silkworm excrement, and remove diseased silkworms and immature silkworms as materials, taking 10Kg of materials as an example.

[0045] 2. Spread the cooked silkworms on a plastic net, and spray it with 50°C warm water through a sprayer to achieve the cleaning effect.

[0046] 3. Put the washed cooked silkworms into a container of about 40Kg of normal temperature water, treat them with a square ultrasonic probe, ultrasonic frequency 30 kHz, power 300 watts, time 30 minutes, temperature 20 ℃, induce the production of antimicrobial peptides.

[0047] 4. Pick up the cooked silkworms after induction, dry them in the air, and place them in a sterile room at 25°C and a relative humidity of 80% to allow t...

Embodiment 2

[0081] The present invention induces production of antimicrobial peptides and processing methods thereof on tussah silkworm chrysalis, specifically comprising the following steps:

[0082] 1. Collection of tussah silkworm chrysalis: cut the tussah silkworm cocoons in autumn to obtain diapause pupae, and remove diseased and dead pupae. Use 100Kg fresh pupa as material.

[0083] 2. Ultrasound-induced antimicrobial peptides, spread silkworm chrysalis on a plastic net, spray and wash with warm water at 50°C, put it into a container of about 700 liters, and inject it with tap water at normal temperature. The ultrasonic generator was purchased from the square ultrasonic probe of Acason Company. Ultrasonic waves are generated after electrification, and an ultrasonic probe is placed on each of the four walls of the container to ensure that the ultrasonic waves spread evenly in the container. The ultrasonic frequency is 25 kHz, the power is 250 watts, and the continuous treatment is 1...

Embodiment 3

[0088] The processing method of tussah silkworm chrysalis serum dry powder of the present invention specifically comprises the following steps:

[0089] 1. Collection of tussah silkworm chrysalis: cut the cocoons of tussah silkworms in autumn to obtain diapause pupae, and remove sick pupae and dead pupae. Use 100Kg fresh pupa as material.

[0090] 2. Ultrasound-induced antimicrobial peptides. Lay silkworm chrysalis on plastic nets, spray and wash them with warm water at 50°C, put them into a container of about 700 liters, and inject them with tap water at normal temperature. The ultrasonic generator was purchased from the square ultrasonic probe of Acason Company. Ultrasonic waves are generated after electrification, and an ultrasonic probe is placed on each of the four walls of the container to ensure that the ultrasonic waves spread evenly in the container. The ultrasonic frequency is 25 kHz, the power is 250 watts, and the continuous treatment is 25 minutes at a temperatur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of Cecropin production by inducing silkworm or tussah pupa, in particular to silkworm powder or pupa powder containing the silkworm Cecropin, a preparation method of pupa serum powder and application thereof. The preparation method comprises the following steps: taking the matured silkworm, unsilking larvae at the end of the 5th instar and the tussah pupa as raw materials, inducing to produce the Cecropin by using ultrasnic wave, producing the silkworm body\pupa body\pupa serum containing the Cecropin into dry powder by using the spray drying technique, and keeping the sterilization activity of the Cecropin to reach a level of 300-3000unit / g. The silkworm powder or pupa powder containing the Cecropin after oral taking can inhibit the multiplication of duck hepatitis B virus (DHBV) DNA in a body of duck, and the inhibition ratio reaches more than 80 percent. The silkworm powder, the pupa powder or pupa serum powder containing the Cecropin are taken as a Chinese medicinal raw material and prepared into capsules for treating hepatitis.

Description

technical field [0001] The invention relates to the technical field of inducing silkworm or tussah silkworm chrysalis to produce antimicrobial peptide (cecropin); in particular, it relates to a preparation method and application of silkworm powder or chrysalis powder and chrysalis serum powder containing silkworm chrysalis antimicrobial peptide. Background technique [0002] According to the data of GenBank, more than 1200 kinds of insect and frog antimicrobial peptides have been published, including more than 50 kinds of silkworm and tussah silkworm antimicrobial peptides. Antimicrobial peptides induced from silkworm or tussah chrysalis can be directly used as antibacterial drugs. Usually, to induce silkworm or tussah silkworm chrysalis to produce antimicrobial peptides is to inject Escherichia coli or Enterobacter cloacae into the body, and produce antimicrobial peptides in vivo after a period of incubation. Because the injection of Escherichia coli will inevitably cause ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/64A61P1/16A61P31/20
Inventor 黄自然黄亚东苏志坚邓小娟项琪
Owner BIOPHARM RES & DEV CENT JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com