Application of tunicamycin in preparing tumour cell reversing drug
A tunicamycin and drug technology, applied in the field of medicine, can solve the problems of blank clinical research and weak basic research field, and achieve the effect of exact effect, small impact and improvement of living conditions.
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Embodiment 1
[0019] Example 1: U266 myeloma cells treated with tunicamycin
[0020] Types and sources of reagents: tunicamycin was purchased from Sigma Company in the United States, loan number T7765, made into 5 mg / mL mother solution with DMSO, stored at 4°C for later use, and diluted with RPMI-1640 culture solution containing 10% fetal bovine serum to the required concentration The required concentration (0.25 μg / ml or 0.5 μg / ml), wherein the content of DMSO is lower than 0.1%, the effect of DMSO on cells can be neglected under this concentration condition (see literature Hou J, Xiong H, Gao W, Jiang H.2-Methoxyestradiol at low dose induces differentiation of myeloma cells. Leuk Res. 2005; 29: 1059-1067, under this low concentration condition, the effect of DMSO on cells is negligible). The Elisa Kit for detecting the λ chain content in the supernatant was produced by Bethy Company in the United States, the loan number is E80-116, and it was operated according to the instructions. CD49e...
Embodiment 2
[0024] Example 2: RPMI-8226 myeloma cells treated with tunicamycin
[0025] The kind and source of reagent: with embodiment 1.
[0026] Cells and culture method: the myeloma cell line RPMI-8226 was purchased from the Chinese Academy of Sciences, and the rest were the same as in Example 1.
[0027] The method of the experiment: the cells in the proliferating phase were taken for the experiment, and the inoculated cell density was 1×10 5 / L, the concentration of tunicamycin was set to 0, 0.25 μg / ml, and 0.5 μg / ml. After culturing for 48 hours and 72 hours respectively, the supernatant was collected by centrifugation, and the content of the supernatant chain was measured with an Elisa kit. After Wright's staining, the morphological changes were observed; a part of the cells were labeled with CD49e and flow cytometry was used to detect the expression of CD49e.
[0028] Results: Compared with the control cells, after the myeloma cell lines were treated with low dose of tunicamyci...
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