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Method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes

A technology of cyclic adenosine monophosphate and red dates, which is applied in the field of food processing, can solve the problems of long cycle, complicated process, and low product purity, and achieve the effect of large processing capacity, simple process, and high purity of finished products

Inactive Publication Date: 2011-08-10
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies in the prior art, provide a kind of method that extracts cyclic adenosine monophosphate (cAMP) from jujube, to obtain a kind of natural, nontoxic cyclic adenosine monophosphate (cAMP) product, solve existing Complicated process, long cycle, low product purity and other problems, to meet the needs of large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting cyclic adenosine monophosphate (cAMP) from jujube, the steps are:

[0025] (1) Wash 50kg of red dates, soak them in 300L of water at room temperature for 16 hours; precook them for 30 minutes, beat them with a beater, and achieve the separation of pulp and nucleus;

[0026] (2) The slurry is transferred to the extraction tank, 0.5 g / L of pectin mixed enzyme of 6:2:2 is added at 55° C., and the mixture is incubated for 3 hours for enzymolysis and extraction, and the filter press is filtered to obtain jujube juice, and the filter residue is used for recycling production; The function of pectinase is to hydrolyze viscous impurities such as protopectin and pectin into low-molecular dimers and monomers to facilitate the extraction and separation of cyclic adenosine monophosphate (cAMP) in red dates; When the enzyme acts on pectin, it can be hydrolyzed into methanol and pectinic acid. Therefore, the dosage of pectinase should be accurately controlled a...

Embodiment 2

[0033] A method for extracting cyclic adenosine monophosphate (cAMP) from jujube, the steps are:

[0034] (1) Wash 50kg of red dates, soak them in 400L of water at room temperature for 12 hours; precook them for 30 minutes, beat them with a beater, and achieve the separation of pulp and nucleus;

[0035] (2) The slurry is transferred to an extraction tank, 0.3 g / L of pectin mixed enzyme of 6: 2: 2 is added at 60° C. to insulate for 2 hours for enzymolysis and extraction, and the filter press is filtered to obtain jujube juice, and the filter residue is used for recycling production;

[0036] (3) Transfer the jujube juice into a stirring tank to carry out specific adsorption and combination with 8.5kg of activated anion resin, and continuously stir at 40 rpm for 6 hours, filter through 100 mesh to obtain cyclic adenosine monophosphate (cAMP) in the jujube juice combined ) resin, and wash the resin with water about 2 times the amount of the resin to clean the flocs it carries; ...

Embodiment 3

[0041] A method for extracting cyclic adenosine monophosphate (cAMP) from jujube, the steps are:

[0042] (1) Wash 50 kg of red dates, soak them in 350 L of water at room temperature for 14 hours; pre-cook them for 30 minutes, beat them with a beater, and achieve the separation of pulp and nucleus;

[0043] (2) The slurry is transferred to an extraction tank, 0.4 g / L of pectin mixed enzyme of 6: 2: 2 is added at 60° C. to insulate for 3 hours for enzymolysis and extraction, and the filter press is filtered to obtain red date juice, and the filter residue is used for recycling production;

[0044] (3) Transfer the jujube juice into a stirring tank to carry out specific adsorption and combination with 10 kg of activated anion resin, and continuously stir at 40 rpm for 6 hours, filter through 100 mesh to obtain cyclic adenosine monophosphate (cAMP) in the jujube juice combined resin, and wash the resin with water about 2 times the amount of the resin to clean the flocs it brings;...

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Abstract

The invention relates to a method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes, which comprises the steps of cleaning of the red jujubes, soaking of the red jujubes at normal temperature, precooking and pulping, zymohydrolysis on pectin mixed enzyme, filter pressing, anion resin combination, formic acid elution, nano-filtration and interception of formic acid with nano-filtration membrane, and purification, thus obtaining a finished product. The method solves the problems of complex current process, long period, low product purity and the like, and has the advantages of rich raw material sources, simple process, large processing amount, high extraction rate, high product purity and the like; the product is a natural non-toxic product; the finished product cAMP canbe used for medical raw materials or used as food additive; the extracted byproduct red jujube juice can be continuously and deeply processed to promote the industrial chain of comprehensive development and achieve pollution-free production; the modern nano-filtration technology is applied to industrial production, therefore, not only the cost is reduced, but also a better path for deeply processing the red jujube raw material is provided, and the consumption of energy is greatly reduced; and the method not only has important economic benefit, but also has remarkable social benefits.

Description

technical field [0001] The invention belongs to the field of food processing, and relates to a method for extracting, concentrating, crystallizing and purifying active ingredients from natural plants by using modern nanofiltration technology, especially a method for extracting cyclic adenosine monophosphate (cAMP) from jujube. Background technique [0002] Cyclic adenosine monophosphate (cAMP) is composed of adenine, ribose and phosphoric acid. The phosphate group in the molecule and the 3' and 5' hydroxyl groups of ribose form a ring structure of two ester bonds. The molecular weight is 329.21. As we all know, cyclic adenosine monophosphate (cAMP) is the second messenger of certain hormones, which has a variety of physiological functions and pharmacological effects. It can activate certain enzymes involved in the oxidation process of substances in the body, thereby regulating the substance metabolism and energy metabolism of the myocardium; when the body's cardiomyocyte cyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/213C07H1/08B01D61/14
Inventor 王春霞赵丽路福平杜连祥李玉
Owner TIANJIN UNIV OF SCI & TECH
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