Htsnps for determining a genotype of cytochrome P450 1a2, 2A6 and 2D6, PXR and UPD-glucuronosyltransferase 1A gene and multiplex genotyping methods using thereof

A single nucleotide polymorphism, CYP1A2 technology, applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve the problems of insufficient research

Inactive Publication Date: 2009-09-02
申载国
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  • Abstract
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Problems solved by technology

However, there are not many studies on genetic variants in the genes of Koreans, their corresponding haplotypes, and htSNP selection according to each haplotype

Method used

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  • Htsnps for determining a genotype of cytochrome P450 1a2, 2A6 and 2D6, PXR and UPD-glucuronosyltransferase 1A gene and multiplex genotyping methods using thereof
  • Htsnps for determining a genotype of cytochrome P450 1a2, 2A6 and 2D6, PXR and UPD-glucuronosyltransferase 1A gene and multiplex genotyping methods using thereof
  • Htsnps for determining a genotype of cytochrome P450 1a2, 2A6 and 2D6, PXR and UPD-glucuronosyltransferase 1A gene and multiplex genotyping methods using thereof

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Embodiment Construction

[0098] Exemplary embodiments of the present invention will be described below with reference to the accompanying drawings, in which like reference numerals denote like elements, and repetitive description will be avoided as much as possible.

[0099] The present invention can be used to determine the genotype of the CYP1A2 gene found in the Korean people through the variant analysis of the Korean CYP1A2 gene, select htSNP as the optimal signature set for each haplotype, and confirm its availability. In addition, the present invention allows for the determination of novel haplotypes of the human CYP1A2 gene.

[0100] The method for selecting the htSNP of the human CYP1A2 gene of the present invention is as follows:

[0101] (a) collecting a biological sample from a subject;

[0102] (b) extract nucleic acid from the sample collected in operation (a);

[0103] (c) performing PCR with primers that use the nucleic acid extracted in operation (b) as a template to amplify the huma...

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Abstract

The present invention relates to htSNPs for determining a genotype of cytochrome P450 1A2 (CYP1A2), 2A6 (CYP2A6) and 2D6 (CYP2D6), PXR and UDP- glucuronosyltransf erase Ia (UGTlA) genes and a gene chip using the same, and more particularly, to a selection method of htSNPs for determining a haplotype of human CYP1A2, CYP2A6, CYP2D6, PXR and UGTlA genes, a method of determining a genotype of the genes by using the htSNPs and a gene chip therefor.

Description

technical field [0001] The apparatus and method of the present invention relate to haplotype signature single nucleotide polymorphisms (htSNPs) and Gene chip, more specifically, relates to a selection method of htSNP for determining the haplotypes of human CYP1A2, CYP2A6, CYP2D6, NR1I2 (=PXR) and UGT1A genes, a method of determining the genotype of the genes and a method for determining the genotype of the genes The gene chip of the method described above. Background technique [0002] Because of genetic diversity, individuals respond differently to the toxicity and effects of drugs. Therefore, it is necessary to consider the effect of important pharmaceutical proteins relative to genetic diversity during the initial development stage of drugs, because this can reduce the possibility of drug development failure. Haplotype is one of the factors determining genetic diversity among individuals. Haplotype refers to the combination of polymorphisms per gene sequence in a singl...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6827C12Q1/6883C12Q2600/156C12Q2600/172
Inventor 申载国张仁珍李相燮郑惠恩车仁浚金佑永芮晟洙金垠泳车恩暎孙知弘崔银贞金江美郑玄朱
Owner 申载国
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