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Method for detecting and absorbing heavy metal ions in water by bovine serum albumin sensor

A technology of bovine serum albumin and heavy metal ions, applied in the field of adsorption of heavy metal ions in water, bovine serum albumin sensor detection, can solve problems such as threats to health, life and safety, and achieve the effect of low cost and simple preparation process

Inactive Publication Date: 2009-09-02
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The bovine serum albumin sensor can realize real-time detection and adsorption of heavy metal ions in water, which solves the problem that heavy metal pollution has always been a problem for human beings; because heavy metals can enter the human body through breathing, food chain, skin contact, etc., because heavy metals cannot be biologically Degradation, heavy metals in the human body are continuously enriched, posing a serious threat to human health and even life safety

Method used

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  • Method for detecting and absorbing heavy metal ions in water by bovine serum albumin sensor

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Experimental program
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Effect test

Embodiment 1

[0027] a. Add 3.7513g of Al(NO 3 ) 3 9H 2 O and 5.1280g of magnesium nitrate (Mg(NO 3 ) 2 ·6H 2 O) Dissolve in 60 mL deionized water. Slowly add a certain concentration of NaOH solution to excess lactic acid dropwise to adjust the pH of the system to 6-11; add Mg / Al mixed salt solution, continue to drop NaOH solution to pH 6-11, heat to reflux and crystallize 7 -12 hours, a white slurry was obtained, which was centrifuged, washed and dried. Take the above-mentioned lactic acid intercalated hydrotalcite and wash it to neutrality, disperse it in a certain volume of water, and heat it to reflux until it is peeled off;

[0028] b. Under the condition of pH 6.0, prepare 30ml of bovine serum albumin solution with a concentration of 2mg / ml, add 10ml of hydrotalcite nanosheets, and assemble for 1h;

[0029] c. Immersing the treated substrate in the above reaction system for 30 minutes, taking it out for washing and drying to obtain a bovine serum albumin sensor;

[0030] d. Th...

Embodiment 2

[0032] a. Add 3.7513g of Al(NO 3 ) 3 9H 2 O and 5.1280g of magnesium nitrate (Mg(NO 3 ) 2 ·6H 2 O) Dissolve in 60 mL deionized water. Slowly add a certain concentration of NaOH solution to excess lactic acid dropwise to adjust the pH of the system to 6-11; add Mg / Al mixed salt solution, continue to drop NaOH solution to pH 6-11, heat to reflux and crystallize 7 -12 hours, a white slurry was obtained, which was centrifuged, washed and dried. Take the above-mentioned lactic acid intercalated hydrotalcite and wash it to neutrality, disperse it in a certain volume of water, and heat it to reflux until it is peeled off;

[0033] b. Under the condition of pH 7.0, prepare 30ml of bovine serum albumin solution with a concentration of 1.5mg / ml, add 10ml of hydrotalcite nanosheets, and assemble for 1h;

[0034] c. Immersing the treated substrate in the above reaction system for 30 minutes, taking it out for washing and drying to obtain a bovine serum albumin sensor;

[0035] d. ...

Embodiment 3

[0037] a. Add 3.7513g of Al(NO 3 ) 3 9H 2 O and 5.1280g of magnesium nitrate (Mg(NO 3 ) 2 ·6H 2 O) Dissolve in 60 mL deionized water. Slowly add a certain concentration of NaOH solution to excess lactic acid dropwise to adjust the pH of the system to 6-11; add Mg / Al mixed salt solution, continue to drop NaOH solution to pH 6-11, heat to reflux and crystallize 7 -12 hours, a white slurry was obtained, which was centrifuged, washed and dried. Take the above-mentioned lactic acid intercalated hydrotalcite and wash it to neutrality, disperse it in a certain volume of water, and heat it to reflux until it is peeled off;

[0038] b. Under the condition of pH 5.0, prepare a bovine serum albumin solution with a concentration of 3 mg / ml;

[0039] c. Immerse the treated substrate into 50ml hydrotalcite nanosheets for 30 minutes, take out, wash and dry;

[0040] d. Immerse the substrate pre-coated with a layer of hydrotalcite film into a 2mg / ml bovine serum albumin solution for 3...

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Abstract

A method for detecting and absorbing heavy metal ions in water by a bovine serum albumin sensor belongs to the technical field of biologic-inorganic hybridization. The bovine serum albumin sensor is made by automatically assembling bovine serum albumin and houghite nano-sheets, wherein a bovine serum albumin molecule contains rich fluorescence-emission groups such as tryptophan, tyrosine and phenyl alanine, the heavy metal ions cause the fluorescence of the bovine serum albumin to quench, and the fluorescence intensity change of the bovine serum albumin and the quantity of the fixed heavy metal ions are in linear relation; the fluorescence of the bovine serum albumin molecule quenches regularly along with the increase of the concentration of the heavy metal ions in water, and the fluorescence intensity change of the bovine serum albumin and the initial concentration of the heavy metal ions in water are in fine linear relation. The bovine serum albumin sensor can perform real-time and on-line detection and absorption to the heavy metal ions in water within the concentration range of 1-100ppm of the heavy metal ions.

Description

technical field [0001] The invention belongs to the technical field of biological-inorganic hybridization, and in particular relates to a method for bovine serum albumin sensor detection and adsorption of heavy metal ions in water. Background technique [0002] Bio-inorganic nano-hybrid materials based on the assembly of active biomolecules and inorganic nano-materials have the unique properties of both, and have become research hotspots in the fields of life science, material science, and nanoscience. Starting from inorganic materials, inorganic-biological hybrid materials are mainly reflected in the following three aspects: (1) Inorganic-biological hybrid materials formed based on zero-dimensional nanoparticles. Semiconductor nanoparticles such as (CdS, CdSe, ZnS, TiO 2 etc.) or metal nanoparticles (such as Au) can be coupled with enzymes, antigens, antibodies or nucleic acids such as DNA to achieve functionalization, and can also be assembled into a certain arrangement u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C01F7/02
Inventor 何静周良彪
Owner BEIJING UNIV OF CHEM TECH
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