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CXCL10 gene polymorphism relevant to progression of chronic hepatitis type B disease course and detection method thereof

A chronic hepatitis B, gene technology, applied in the genotype detection of the susceptibility gene CXCL10, the detection kits for the genotypes of the susceptibility genotypes mentioned above, can solve the problem of changing expression or binding activity, affecting liver damage and disease severity, etc. problems to reduce the risk of occurrence

Inactive Publication Date: 2009-09-02
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene may alter its expression or binding activity, thereby affecting liver damage and disease severity

Method used

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  • CXCL10 gene polymorphism relevant to progression of chronic hepatitis type B disease course and detection method thereof
  • CXCL10 gene polymorphism relevant to progression of chronic hepatitis type B disease course and detection method thereof
  • CXCL10 gene polymorphism relevant to progression of chronic hepatitis type B disease course and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this example, a set of primer pairs was designed and synthesized, and based on the primer pairs, a method for detecting the G-201A genotype of the CXCL10 gene associated with the progression of hepatitis B was designed.

[0027] 1. Genomic DNA extraction

[0028] Genomic DNA of peripheral blood leukocytes was extracted by the phenol / chloroform method in the field.

[0029] 1) Take sodium citrate or EDTA-Na 2 5ml of anticoagulated whole blood (try not to use heparin for anticoagulation), put in a 50ml capped centrifuge tube;

[0030] 2) Add 3-5 times the volume of cold distilled water, invert and mix repeatedly, place in ice for 5 minutes, and centrifuge at 2000rpm at 4°C for 20 minutes;

[0031] 3) Slowly decant the supernatant, add pre-cooled 0.1% Triton-X100 (same volume as above) to the precipitate, gently mix the precipitate, centrifuge as above, and discard the supernatant;

[0032]4) Add 4ml of lysate (50Mm Tris-Cl-10mM EDTA, pH 8.0) to the precipitate, brea...

Embodiment 2

[0046] In this example, the method established in Example 1 was used to analyze the G-201A genotype of the CXCL10 gene of 1147 cases of chronic progressive hepatitis B patients and 1253 cases of asymptomatic HBV carriers.

[0047] 1. Test object:

[0048] This study included 1147 chronic progressive hepatitis B patients and 1253 asymptomatic HBV carriers (collected from Beijing Hospital of Infectious Diseases [613 copies] and Chongqing Southwest Hospital [1787 copies] from February 2001 to March 2006). ]). All HBV-infected patients were positive for HBsAg and anti-HBc IgG for at least 12 months. All carriers underwent liver function test, serum marker detection, ultrasound / computed tomography imaging during the study period, and 376 cases (15.7%) underwent liver biopsy. All subjects had no serological evidence of HCV, HDV, and HIV co-infection. While collecting tissue specimens or blood samples, each research subject signed an informed consent form and provided detailed cli...

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Abstract

The invention relates to a detection method of G-201A locus of predisposing gene CXCL10 relevant to progression of chronic hepatitis type B disease course. The method is a PCR-RFLP method (namely polymerase chain reaction-restriction fragment length polymorphism method). The invention further relates to a kit for detecting the predisposing locus and the application thereof. In addition, by linking the G-201A locus of gene with the occurrence risk of the progression of chronic hepatitis type B disease course, the invention determines CXCL10 as a novel predisposing gene of the progression of chronic hepatitis type B disease course and provides new genetic counseling content for people who are chronically infected with hepatitis type B about the early warning of disease severity.

Description

technical field [0001] The invention relates to the individual susceptibility and genetic detection method of the progress of the disease course of chronically infected patients of hepatitis B virus (HBV). Specifically, the present invention relates to a genotype detection method of the susceptibility gene CXCL10 related to the progress of the disease course of HBV chronic infection patients. The present invention further relates to a detection kit for the above-mentioned susceptibility gene genotype, and the application of the kit. In addition, the present invention also relates to the application of the above-mentioned detection method and susceptibility gene in early warning and genetic counseling of HBV chronically infected patients. Background technique [0002] HBV is the most common causative agent of acute or chronic hepatitis worldwide, especially in some Asian and African countries. During chronic HBV infection, HBV-specific CD8 + T cell immune responses cause l...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 贺福初周钢桥翟芸杨灏
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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