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Method for screening pseudomonas syringae pv.tabaci antagonistic bacteria

A technology for germs and wildfires, applied in the field of biological control, can solve the problems of low screening efficiency and need to be improved, and achieve the effect of wide adaptability and improved screening efficiency.

Active Publication Date: 2009-09-02
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the screening efficiency of these asynchronous confrontation culture methods is low and needs to be improved

Method used

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  • Method for screening pseudomonas syringae pv.tabaci antagonistic bacteria
  • Method for screening pseudomonas syringae pv.tabaci antagonistic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] (1) Cultivation of bacteria to be tested, tobacco wildfire bacteria

[0025] The bacterial strains to be tested are 040239 and 050117, a total of 2 strains, the tobacco wildfire strain pst05, all isolated from tobacco diseased leaves. 5 points of 2 bacterial strains to be tested were spot-connected on NA or KB plates (in this example, 5 points of the same plate were the same strain, but 5 different bacterial strains could be used in specific implementation), tobacco wildfire pst05 was inoculated by streaking, 28 ± 1 Cultivate for 2 to 3 days at ℃;

[0026] (2) Preparation of Tobacco Wildfire Bacteria Suspension

[0027] Take 10-15ml of 0.1% Tween 80 sterilized aqueous solution and add it to the cultivated tobacco wildfire bacteria plate, scrape off the bacterial lawn, transfer it to a sterilized Erlenmeyer flask, and adjust the bacterial concentration with 0.1% Tween 80 sterilized aqueous solution for 10 4～5 cfu / ml, the suspension of tobacco wildfire bacteria can be ...

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Abstract

The invention discloses a method for screening pseudomonas syringae pv.tabaci antagonistic bacteria, which is characterized in that an improved asynchronism opposing culture method is adopted, bacteria to be tested are in point inoculum of 4-6 points and are cultured for 2-3 days, pseudomonas syringae pv.tabaci suspension is sprayed and dispersed, the bacteria are continuously cultured in an opposing way for 2-3 days after the water on the surface is dried in a sterile way and are observed for inhibition zones, and the bacteria with the inhibition zones are target bacteria obtained after the screening. The invention can remarkably enhance the screening efficiency, enrich the asynchronism opposing culture method and accelerate the biological control of bacterial plant diseases.

Description

technical field [0001] The invention relates to biological control technology, more specifically, the invention relates to a method for screening antagonistic bacteria of tobacco wildfire fungus. Background technique [0002] Tobacco wildfire disease is a bacterial disease caused by Pseudomonas syringae pv. It is one of the important bacterial diseases on tobacco in tobacco-producing countries and regions. The disease was discovered in Liaoning smoking area in our country as early as the 1950s, but it has been harmless. Since the 1980s, the disease has occurred in a large area in Yunnan, Guizhou, Henan, Shandong, Hubei, Liaoning and Jilin and other smoking areas, and has a tendency to increase year by year, causing serious economic losses. [0003] Wildfire fungus can overwinter in fields with diseased plant residues, survive in the roots of many other crops and weeds, and can be spread by tobacco seeds. Therefore, it is quite difficult to prevent the occurrence of the di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
Inventor 方敦煌殷端姬广海李光西白江兰
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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