Method of potato virus eradication

A potato virus removal technology, applied in horticultural methods, botanical equipment and methods, plant preservation, etc., can solve the problem of unfavorable survival of shoot tips and regeneration of differentiated plants, low plant survival rate and virus-free rate, and difficulty in cutting The stem tip and other problems can be solved, and the effect of saving manpower and financial resources, less difficult operation and strong frost resistance

Inactive Publication Date: 2009-09-02
HENAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, it is necessary to cut out 0.1-0.5mm shoot apex meristem tissue under an anatomical microscope during shoot tip culture, which is relatively difficult to operate and cultivate, requires high technical experience, and the plant survival rate and virus-free rate are low. The method has the problems of low shoot tip culture survival rate, difficult technical operation, and low removal rate of some viruses that endanger potato production.
The low virus removal rate of the shoot tip detoxification operation is mainly due to the difficulty in grasping the size of the cut shoot tip in operation. Since the transmission of the virus is realized through vascular bundles, some cells in the meristem of the shoot tip are immature. There is no complete vascular bundle, so it is difficult for the virus to reach the shoot tip, so the operation of shoot tip detoxification is relatively high. The cut shoot tip should be small enough, but too small shoot tip is not conducive to survival and regeneration. Intact plants, these are the problems with current shoot tip detoxification methods

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] This embodiment is to test the "Zao Dabai" potato variety. The specific operation method is: select potato materials, carry out multiplication, and then carry out virus detection on the materials, and the method used for detection is the RT-PCR method; then establish a single bud Line materials, the established single-bud line materials are propagated and subcultured, and the materials confirmed to carry the virus are propagated for cryopreservation. The subculture medium is selected: MS basic medium + 0.2mol L -1 6-BA+0.03mol L -1 IAA was cultured for proliferation, the pH of the medium was 5.8, the temperature of the culture room was 22 °C, and the light intensity was 40 μmol m -2 ·s -1 , the light time is 12h·d -1 ; After a certain amount of material has been multiplied, cryopreservation is carried out, that is, the vitrification method is adopted, exercised at a low temperature of 4°C for 6 days, and then pre-cultured in a medium supplemented with dimethyl sulfoxi...

Embodiment 2

[0015] This embodiment is to test "Zhengshu No. 5" potato varieties. The specific operation method is: select potato materials, carry out multiplication, and then carry out virus detection to the materials, and the method used for detection is the RT-PCR method; then establish For single-bud material, the established single-bud material is propagated and subcultured, and the virus-carrying material is confirmed to be multiplied for cryopreservation. The subculture medium is selected: MS basic medium + 0.2mol L -1 6-BA+0.03mol L -1 IAA was cultured for proliferation, the pH of the medium was 5.8, the temperature of the culture room was 23 °C, and the light intensity was 38 μmol m -2 ·s -1 , the light time is 13h·d -1 ; When the material is multiplied to a certain amount, cryopreservation is carried out, that is, the vitrification method is adopted, exercised at a low temperature of 3°C for 7 days, and then placed in a medium supplemented with dimethyl sulfoxide (DMSO) and ace...

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Abstract

The invention discloses a method of potato virus eradication, which includes the following steps: taking a material for virus detection, carrying out the tissue cultivation to determined material with virus, and then establishing a simple-bud material; carrying out reproducing successive-transfer cultivation to the established simple-bud material and ultra-low temperature storage treatment to the simple-bud material, reproducing the survived material after ultra-low temperature storage, and then carrying out virus detection to the survived material; and reproducing the material without virus after detection to be used for producing seed potato without virus. Compared with the traditional stem-tip virus eradication, the method of the invention has lower operation difficulty and higher virus eradication rate, and the virus which can not be easily eradicated by stem-tip cultivation can be eradicated by the method. The invention has excellent social and economic values and wide application prospect.

Description

technical field [0001] The invention relates to a method for removing plant virus, in particular to a method for removing potato virus. Background technique [0002] Potato is an important economic crop. my country has become the world's largest potato producer, with planting area and output accounting for nearly a quarter of the world's total. The potato industry has absorbed nearly 50 million rural laborers and 2 million urban workers. 30% of the population in the west. According to the survey, the annual per capita consumption of potatoes in my country is about 30 kg, which is far below the level of 75 kg per capita in developed countries. There is a large gap in the demand for potato flour and starch, and domestic production can only meet 50% of the demand. Potatoes can be used for processed food, whole There are more than 2,000 kinds of products such as flour, starch and modified starch, and the industrial chain is long, which can realize multiple value-added. Therefore...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01N3/00
Inventor 王子成曲先何艳霞李忠爱
Owner HENAN UNIVERSITY
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