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Tridax procumbens total flavone preparation method and use thereof

A technology for the production of total flavonoids from Miscanthus flavonoids, which is applied in the field of preparation of total flavonoids from Miscanthus flavonoids, which can solve the problems of troublesome operation, large amount of solvent used, low extraction rate and transfer rate of flavonoids, etc.

Inactive Publication Date: 2009-08-19
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods often have the disadvantages of large amount of solvent, long extraction time, troublesome operation, and low work efficiency. The separation and enrichment of flavonoids mostly uses macroporous resin column chromatography, and resin fillers with different performance specifications are selected. Gradient elution with organic solvents
These methods also have the disadvantages of many operating steps and elution steps, large amount of organic solvent, low yield, and high production cost. In particular, there are many heating and concentration links in the extraction and purification process, which may easily cause damage or loss of active ingredients. , the extraction rate and transfer rate of total flavonoids are not high, and harmful solvents such as chloroform, ethyl acetate, n-butanol, and methanol are often used, causing pollution to the environment. At the same time, flavonoid extracts often contain some heavy metal impurities, and often exceed the standard

Method used

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  • Tridax procumbens total flavone preparation method and use thereof
  • Tridax procumbens total flavone preparation method and use thereof
  • Tridax procumbens total flavone preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Raw material processing: crush the whole herb of Pistachio chrysanthemum into coarse powder, and pass through a 40-mesh sieve;

[0026] 2) Extraction: Put the chrysanthemum chrysanthemum powder into the extraction container, put it into an ultrasonic extractor, and use 8 times the amount of 60% aqueous ethanol as a solvent for ultrasonic extraction twice under the condition of 50°C. Each time for 45 minutes, filter with suction, and combine the filtrates;

[0027] 3) Concentration under reduced pressure: Concentrate the filtrate in step 2) with a vacuum film at a water bath temperature below 50° C., recover ethanol, and obtain a concentrated solution;

[0028] 4) Decolorization and degreasing treatment: the concentrated solution of step 3) is ultrasonicated for 40 minutes below 50° C. with powdered activated carbon, the amount of powdered activated carbon is 2% of the weight of Miscanthus coarse powder, suction filtered, activated carbon is removed, and the filtrate ...

Embodiment 2

[0032] In the above step 1): the whole herb of Misantha chrysanthemum is crushed into coarse powder, and passed through a 20-mesh sieve; in the above step 2): the temperature of ultrasonic extraction is 40°C, the time of ultrasonic extraction is 60min, and the amount of hydrous ethanol is 10 times of weight, the concentration of aqueous ethanol is 70%; step 4): in the step 4): the time of ultrasonic decolorization and degreasing treatment is 50min, the consumption of powdered activated carbon is 3% of the weight of the chrysanthemum chrysanthemum powder; in step 6): successively Gradient elution with 20%, 30%, 35%, 55%, 65% aqueous ethanol; the elution flow rate is 15mL·min -1 The diameter-to-height ratio of the chromatographic column is 1:15; the eluent from each part of 30-55% aqueous ethanol is combined; other steps are the same as in Example 1.

Embodiment 3

[0034] In above-mentioned step 2): the temperature of ultrasonic extraction is 60 ℃; Ultrasonic extraction time 30min; 1% of Miscanthus chrysanthemum coarse powder weight; step 6) in: carry out gradient elution with 15%, 25%, 45%, 50%, 60% aqueous ethanol successively; Elution flow rate is 5mL·min -1 ; Merge the eluent of each part of 25-60% aqueous ethanol; Other steps are the same as in Example 1.

[0035] In the above step 2): the temperature of ultrasonic extraction is 45°C, 48°C, 55°C; the time of ultrasonic extraction is 35min, 45min, 55min; the concentration of aqueous ethanol is 55%, 65%. In step 4): the time for ultrasonic decolorization and degreasing treatment is 32 minutes, 35 minutes and 45 minutes. In step 6): the elution flow rate is 6mL min -1 , 12mL·min -1 , 8mL·min -1 . Other steps are the same as in embodiment 1, and can achieve the same technical effect.

[0036] In the above step 2), using 6-8 times the amount of water-containing ethanol as a solvent...

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Abstract

The invention discloses a method for preparing total flavones of Tridax procumbens and application thereof, which belong to the technical field of extraction and preparation methods of flavones. The method comprises the steps of: taking crude powder of the Tridax procumbens as a raw material, using aqueous ethanol as a solvent for ultrasonic extraction, recovering the ethanol by performing vacuum concentration on a filtrate, performing ultrasonic decolorization by activated carbon after obtaining a concentrated solution, continuing to perform vacuum concentration after removing the activated carbon, separating and enriching the concentrated solution through a macroporous absorbent resin, using the aqueous ethanol for gradient elution, and mixing an aqueous ethanol eluent with certain proportion for vacuum concentration drying to obtain dry powder of an extract of the total flavones of the Tridax procumbens. The preparation method has the advantages of simple process, convenient operation, small equipment investment, small using amount of the solvent, no environmental pollution, low production cost, and high yield of the extract.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation methods of flavonoids, and specifically relates to a preparation method and application of total flavonoids of P. Background technique [0002] Flavonoids are a class of important biologically active substances that widely exist in the plant kingdom. They often have anti-oxidation, anti-aging, anti-tumor effects, and have good development and application value. [0003] Tridax procumbens.L is a perennial herbaceous plant of the family Asteraceae. It is native to the tropical regions of America and later spread to countries such as India, Indochina and Indonesia. Our country introduced Hainan, Guangdong and Taiwan and other places. Slightly sweet, bitter cold. The whole herb of P. chinensis has anti-oxidation, anti-tumor, anti-bacterial and anti-inflammatory effects. As for the preparation method and the application of the total flavonoids of P. chinensis, no research report ...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P39/06A61P35/00
Inventor 袁珂
Owner ZHEJIANG FORESTRY UNIVERSITY
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