Endogenetic fungus for producing paclitaxel

An endophytic fungus and paclitaxel technology, applied in the field of paclitaxel-producing fungi, can solve problems such as difficulty in meeting the market and research, few strains, etc.

Inactive Publication Date: 2009-07-22
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the huge medical value of paclitaxel, scholars at home and abroad have joined in the research of paclitaxel-producing microorganisms. However, there are still only a handful of isolated strains that can be used for the microbial fermentation of paclitaxel, which is difficult to meet the needs of the market and research.

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  • Endogenetic fungus for producing paclitaxel
  • Endogenetic fungus for producing paclitaxel
  • Endogenetic fungus for producing paclitaxel

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Experimental program
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specific Embodiment approach 1

[0012] Specific embodiment 1: The endophytic fungus HD86-9 (Aspergillus niger var. taxi HD86-9) that produces paclitaxel in this embodiment belongs to Aspergillus niger var. nov), preserved in China Center for Type Culture Collection, the preservation date is December 25, 2006, and the preservation number is CCTCC M 206137.

[0013] The paclitaxel-producing endophytic fungus HD86-9 has a very high homology with Aspergillus species through 18S rDNA sequence alignment (NCBI database), and the homology is more than 98%, so it is confirmed that the paclitaxel-producing endophytic fungus Phytophyte HD86-9 is closely related to Aspergillus in evolutionary position.

[0014] The phylogenetic tree constructed from the 18S rDNA of the taxol-producing endophytic fungus HD86-9 and similar strains recorded in Genbank is as follows: Figure 5 shown. The phylogenetic tree constructed by the taxol-producing endophytic fungus HD86-9 and the ITS region (including 5.8S rDNA) of similar strain...

specific Embodiment approach 2

[0015] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the paclitaxel-producing endophyte HD86-9 is an endophyte strain in the bark of Taxus cuspidata Sieb. et Zucc. Others are the same as the first embodiment.

[0016] In this embodiment, the endophytic fungus HD86-9 producing paclitaxel is isolated from the bark of Taxus cuspidata Sieb.et Zucc. The endophytic fungus HD86-9 producing paclitaxel in the present embodiment is separated and obtained by the following steps: 1. Rinse the bark of Taxus chinensis with sterile water (to remove impurities such as dust on the surface of the bark), and then wash it with a mass concentration of 75% soak in alcohol for 3 to 10 minutes, then rinse with sterile water (to remove the alcohol), and cut into small pieces of yew bark with an area of ​​0.5cm×0.5cm with sterile scissors; The small pieces are respectively planted on the PDA medium, Chapek (Czapek) medium and Martin (Martin) agar medium plates and then ...

specific Embodiment approach 3

[0018] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the colony texture of the paclitaxel-producing endophytic fungus HD86-9 is fluffy and has radial grooves, and the opposite side of the colony is yellow, and the color gradually changes from outside to inside. Deepen to yellowish brown or dark brown; the paclitaxel-producing endophytic fungus HD86-9 strain has aerial hyphae, the paclitaxel-producing endophytic fungus HD86-9 has a large number of conidia structures, and the conidia heads are carbon black and spherical, The diameter of the conidia head is 150-500 μm, and the apical capsule is spherical or nearly spherical, with a diameter of 30-80 μm. Others are the same as the first or second embodiment.

[0019] The conidia of the paclitaxel-producing endophytic fungus HD86-9 split into several cylindrical structures with diameters up to 800 μm or larger.

[0020] The paclitaxel-producing endophytic fungus HD86-9 was cultured on Saccharom...

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Abstract

The invention discloses a taxol-producing endophytic fungus and relates to taxol-producing fungi. The invention provides a new taxol-producing endophytic fungus and increases the number of strains that can be used in microbial fermentation of taxol. The taxol-producing endophytic fungus HD86-9 belongs to aspergillus niger yew variants and is preserved in China Center for Type Culture Collection with the preservation date of December 25th, 2006 and the preservation number of CCTCC M 206137. The taxol-producing endophytic fungus HD86-9 belongs to a new aspergillus niger yew variant under the analysis of morphology, 18S rDNA sequence and ITS. The invention not only provides an additional taxol-producing endophytic fungus, but also establishes physical foundation for the genetic modification of taxol-producing microorganisms in future.

Description

technical field [0001] The present invention relates to a paclitaxel-producing fungus. Background technique [0002] Malignant tumors seriously threaten human health, and cancer is the second leading cause of death in human diseases after cardiovascular and cerebrovascular diseases. [0003] Paclitaxel is a diterpene alkaloid drug extracted from Taxus genus plants with various anti-tumor effects. Because yew (also known as yew) is a rare tree species, its growth rate is slow, and its biological characteristics have high requirements on the ecological environment, and its natural renewal ability is poor, so the population is extremely limited. It is an endangered plant and its resources are very limited. Therefore, Scholars at home and abroad are actively researching and developing new drug sources of paclitaxel. [0004] Microbial fermentation production is the most ideal way to reduce costs and obtain paclitaxel in large quantities. In 1993, Andrea Stierle, a chemist at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/685
Inventor 赵凯周东坡平文祥李强
Owner HEILONGJIANG UNIV
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