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Small molecule peptide and use thereof

A technology of small molecule peptides and beads, applied in the field of organic chemistry, can solve problems such as unclearness, and achieve the effect of improving the diagnosis rate

Inactive Publication Date: 2009-07-22
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, A549 is only one type of lung cancer cells. Whether this small molecular peptide can specifically bind to cancer cells in the pleural effusion of lung cancer patients is still unclear.

Method used

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  • Small molecule peptide and use thereof
  • Small molecule peptide and use thereof
  • Small molecule peptide and use thereof

Examples

Experimental program
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Effect test

Embodiment Construction

[0015] 1, utilize solid-phase synthesis method to prepare resin beads of the present invention

[0016] (1) Weigh 1g of NH on the surface 2 Resin beads of active groups, washed 3 times with dimethylamide, and then soaked in dimethylamide until the beads are fully swollen;

[0017] (2) Add cysteine ​​and N, N'-diisopropylcarbodiimide, react at room temperature for 2 hours, wash the beads 5 times with DMF, then add 20% piperidine, and react at room temperature for 5 minutes , then add 20% DMF, react for 15min, and completely remove the Fmoc protecting group;

[0018] (3) sequentially coupling aspartic acid, glycine, isoleucine, glycine, proline, glutamine and cysteine ​​according to step (2);

[0019] (4) Wash the beads connected with the last amino acid once with 25% trifluoroacetic acid and three times with distilled water to obtain the resin beads of the present invention.

[0020] 2. Obtaining the small molecule peptide of the present invention

[0021] The small molecul...

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Abstract

The invention provides a small molecule peptide with the amino acid sequence of cdGIGPQc (SEQ NO.1); and the invention can be prepared by a solid-phase synthesis method. The small molecule peptide has specific adhesion action to non-small cell lung cancer cells and can be used for separating the non-small cell lung cancer cells. The invention also provides a resin bead which is formed by a resin bead with an NH2 active group on the surface and the small molecule peptide by -CO-NH- connection. The resin bead has very high specificity for the non-small cell lung cancer cells, can be used for assisting chest hydrocele desquamate cell test method in diagnosing non-small cell lung cancer, and improves the diagnosis rate to be 13.51%.

Description

technical field [0001] The invention relates to the field of organic chemistry, in particular to a peptide, especially a peptide containing 5-11 amino acids. Background technique [0002] Lung cancer has ranked first in the incidence of nausea and tumors in urban my country, and its incidence is still showing an obvious upward trend. Pleural effusion often occurs due to pleural metastasis of lung cancer, so pleural effusion exfoliated cell examination is a commonly used diagnostic method. Centrifuge 1ml of cells; ②Use a pipette to pipette about 50 μl and drop it on the glass slide, and scrape it with another glass slide; ③After drying, fix with 75% alcohol + formaldehyde for 10 minutes; ④Stain with hematoxylin for 1 minute; ⑤Stain with eosin for 1 minute ; ⑥ rubber sealing film. However, this method is not sensitive. Finding cancer cells in a large amount of pleural effusion is like looking for a needle in a haystack. Moreover, the cells in pleural effusion are mixed, incl...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N5/08C07K17/06
Inventor 郭琳琅郭颖张帆蔡颖谦
Owner SOUTHERN MEDICAL UNIVERSITY
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