Biocontrol fungicide suitable for stalk returning of wheat and corn and soil-borne disease control as well as preparation method thereof
A technology of corn stalks and biocontrol fungicides, which is applied in the directions of botanical equipment and methods, biocides, fungicides, etc., can solve the problems of wheat sowing, adverse effects on growth, slow decomposition speed, and poor operation efficiency of plough layer soil structure. , to achieve the effects of human and animal safety, improvement of soil properties, and convenient application
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[0076] The production process of the biological preparation of the present invention is further illustrated by the following examples (see accompanying drawing).
[0077] Dry the corn stalks, crush them into powder by machine, add wheat bran and corn flour in a fixed ratio (weight ratio) (straw flour:wheat bran:corn flour=4:3:2), and then add nutrient solution (glucose 10g , MgSO 4 1g, CaCl 2 1g, (NH 4 ) 2 SO 4 15g, distilled water 1000mL) fully stir evenly, make water content reach 200% (volume: weight). After bagging, it is sterilized by high pressure and wet heat (121°C for 60 minutes) to make sterile solid compost.
[0078] Activate the original strain of B1514, streak culture on LB medium plate, and culture at 28°C for 18h. After the cultivation, add 5ml of sterile water to each petri dish, spread it evenly to make a bacterial suspension, put it into liquid LB medium according to the inoculum amount of 2%, carry out shaking table cultivation, and cultivate at 200...
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