Tissue culture, seedling cultivation and rooting method of Fructus schisandrae
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A technology of tissue culture and Schisandra chinensis, applied in the field of plant propagation, to achieve the effects of low production cost, high seedling rate and large reproduction coefficient
Active Publication Date: 2011-01-19
朱俊义
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Wu Xiuju et al. reported the tissue culture of the medicinal plant Schisandra chinensis in 1999, using the tender stems with axillary buds as explants, using MS medium as the basal medium, and adding 6-BA 2.0 +ZT 0.1 mg / L, the effect of inducing buds is good; with the addition of 6-BA 2.0 +NAA 0.2 +ZT 0.1 mg / L, the proliferation rate of the buds is the best; the rooting effect is the best when adding 0.2mg / L NAA, but the experiment with the data provided by the relevant information did not reproduce the results
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[0011] 1. Utilize conventional tissue culture techniques (such as axillary bud cluster differentiation or callus differentiation) to obtain clustered seedlings of Schisandra chinensis, and intercept the stem segments.
[0012] 2. Pretreatment and rooting culture of clustered seedlings
[0013] Effects of cross-matching of different concentrations of plant growth regulators in 1 / 8MS medium on rooting of stem segments of Schisandra chinensis pretreated
[0014] 1 Materials and methods
[0015] 1.1 Screening of sprout rooting pretreatment medium
[0016] 1 / 8MS was used as medium, and different concentrations of IAA, IBA and KT were added. Sucrose 10g / L, agar 8.0g / L, pH6.5, screen the most suitable pretreatment medium for rooting.
[0017] Culture conditions: temperature (22±2)°C, dark treatment for 48 hours.
[0018] 1.2 Sand rooting
[0019] Take out the tender stem section after 48h dark treatment from...
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Abstract
The invention relates to plant propagation technology, in particular to a schizandra chinensis tissue culture seedling raising and rootage method. The method comprises the following steps: obtaining clustered seedlings through the tissue culture technology; intercepting the stems of the seedlings and putting the stems in a rootage culture medium for pretreatment; then taking root in a substrate. The method is characterized by quick propagation, high propagation coefficient, convenient management, low production cost, high stocking percent, short culture cycle, anniversary production and industrialization promotion.
Description
technical field [0001] The invention relates to a plant propagation technology, that is, a method for cultivating seedlings and rooting of Schisandra chinensis tissue culture. Background technique [0002] In the prior art, Schisandra chinensis (Turcz) Baill, also known as Schisandra chinensis (Turcz) Baill, is a perennial deciduous woody vine of the Magnoliaceae Schisandra genus, and its fruit and seeds are famous traditional Chinese medicines, which can be used to treat neurasthenia, fatigue, Night sweats, etc.; stem bark can be used as seasoning, spices, and juice can be used to make beverages, fruit wine, etc. The length of Schisandra chinensis can reach more than 10m, the stem skin is gray-brown, the skin is obvious, the branchlets are brown, slightly ribbed, the leaves are alternate, thin, slightly membranous, the stalk is slender, the leaves are oval, 5-11cm long, and wide 3-5cm, apex acute or acuminate, base cuneate, margin sparsely cusps, upper part dark green, shi...
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