Method for preparing butanoic acid from mixed fermentation ligno-cellulose

A technology for lignocellulose and mixed bacteria fermentation, which is applied in the field of butyric acid biological fermentation, can solve the problems of increasing product cost, prolonging fermentation time, waste of raw materials, etc., and achieves the effects of reducing raw material cost, reducing inhibition and avoiding cost.

Inactive Publication Date: 2009-07-08
青岛生物能源与过程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] First, commercial cellulase is expensive, which reduces the price competitiveness of the product
[0007] Second, for hexoses and pentoses, the strains can only use one of them;
It causes waste of raw materials, prolongs the fermentation time, and increases the product cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of seed medium

[0030] Prepare liquid seed medium on the basis of Clostridium medium, and the specific composition is as follows:

[0031] Add to 1L medium: 1.3g (NH 4 ) 2 SO 4 , 2.60gMgCl 2 .6H 2 O, 1.43gKH 2 PO 4 , 7.2gK 2 HPO 4 .3H 2 O, 0.13g CaCl 2 .2H 2 O, 6g sodium glycerophosphate, 11mg FeCl 2 .7H 2 O, 5g yeast extract, 10g sugar (Clostridium thermocellum-crystalline cellulose, Clostridium thermobutyricum-glucose), 1mg resazurin, 0.1g cysteine. The pH was adjusted to 7.0, and N 2 :H 2 :CO 2 =80:5:15 to maintain the anaerobic state of the medium, and sterilize at 121°C for 20 minutes. Culture medium preparation, inoculation, and sampling were all performed at an anaerobic workstation.

[0032] 2. Prepare fermentation medium, acid-treated straw 60g, urea 5g, KH 2 PO 4 1g, K 2 HPO 4 1g, 1000ml water, pH7.2.

[0033] 3. Fermentation process

[0034] (1) Inoculate Clostridium thermocellum and Clostridium thermocellum on solid s...

Embodiment 2

[0039] The preparation of the seed medium is the same as in Example 1.

[0040] Preparation of fermentation medium, 60g of ammonia blasted straw, 5g of urea, KH 2 PO 4 1g, K 2 HPO 4 1g, 1000ml water, pH7.2.

[0041] Put 1000ml of fermentation medium into a fermenter and sterilize at 121°C for 20 minutes. Add Clostridium thermocellum seed culture medium to the fermenter with an inoculum size of 10%, and perform anaerobic liquid fermentation. After 24 hours, add the Clostridium thermophilicum seed culture medium to the fermenter with an inoculum size of 10%, and ferment The temperature is 55°C, and the fermentation time is 3 days. Samples were taken every 6 hours to determine the concentration of butyric acid.

[0042] The method for detecting the concentration of butyric acid is as in Example 1. After continuous fermentation for 72 hours, the final concentration of butyric acid was 15.8g / l.

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Abstract

A method for preparing butanoic acid using bacteria mixing fermentation lignocellulose adopts the thermal fiber clostridium and thermophile butanoic clostridium, and takes the clostridium culture medium as medium, to formulate the seed culture medium; takes the preprocessed plant straw as raw material, adding the nitrogen source and inorganic salt to formulate a fermentation medium. The method includes main steps as follows: A, inoculating the thermal fiber clostridium and thermophile butanoic clostridium on a solid culture medium to perform anaerobic cultivation; B, respectively inoculating the thermal fiber clostridium and thermophile butanoic clostridium cultured by the step A onto a liquid seed culture medium; C, adding the thermal fiber clostridium seed culture medium in the step B in the fermentation medium for perform anaerobic liquid state fermentation; D. adding the thermophile butanoic clostridium seed culture medium in the step B in the fermentation tank, mixing evenly to perform high temperature bacteria mixing anaerobic fermentation to prepare the butanoic acid.

Description

technical field [0001] The invention relates to a method for preparing butyric acid by biological fermentation, in particular to a method for preparing butyric acid by fermenting lignocellulose with mixed bacteria. Background technique [0002] Butyric acid is widely used in food, chemical and pharmaceutical industries. Butyrate can be used as a food additive to increase food flavor, and high-purity butyric acid is used in the dairy industry to increase the flavor of dairy products. β-hydroxybutyrate can be used to produce degradable poly-β-hydroxybutyrate. Butyric acid has been widely recognized for its efficacy in treating blood disorders, cancer, and gastrointestinal disorders. [0003] Butyric acid is mainly produced by chemical method using petroleum as raw material. However, as people's requirements for food and drug safety and green chemicals are increasing year by year, it is difficult for butyric acid synthesized by chemical methods to enter the food and pharmace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/52C12R1/145
Inventor 张春辉马玉久杨芳晓张跃冬杨华
Owner 青岛生物能源与过程研究所
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