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Tissue regeneration membrane with bioactivity and preparation method thereof

A tissue regeneration membrane and biological activity technology, applied in the direction of prosthesis, surgery, medical science, etc., can solve the problems of easy acid production, inflammatory reaction in the implanted area, rejection reaction, etc., achieving easy control, simple preparation method, suitable for wide range of effects

Inactive Publication Date: 2009-07-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these degradable biofilms are composed of collagen and polylactic acid, etc., but after they are implanted in the body, they are prone to produce acid during the degradation process, which may lead to inflammatory reactions in the implanted area, and there is a risk of rejection in the body

Method used

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  • Tissue regeneration membrane with bioactivity and preparation method thereof
  • Tissue regeneration membrane with bioactivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Bioactive Tissue Regeneration Membrane for Periodontal Tissue Regeneration

[0022] (1) Select clean and decontaminated silk in 0.5% Na 2 CO 3 In aqueous solution, degumming twice at 100°C, 1 hour each time, bath ratio 1:50. The degummed silk fibroin was dried in a drying oven at 80°C until dry. Take 10 grams of degummed silk fiber and dissolve it in 100ml of calcium chloride / ethanol / water ternary solution (the molar ratio of calcium chloride, ethanol, and water is 1:2:8), and dissolve it with magnetic stirring at a constant temperature of 70°C . The silk fibroin solution obtained above is dialyzed, desalted and purified to obtain a water-soluble silk fibroin solution with a concentration of 2.5-3.5%, which is concentrated and dried to obtain silk fibroin.

[0023] (2) Weigh 6g of silk fibroin with a balance, add it to 20ml of deionized water, swell at room temperature, stir and dissolve for 1-3 hours until the silk fibroin is completely dissolved, defoam...

Embodiment 2

[0024] Example 2: Bioactive Tissue Regeneration Membrane for Bone Tissue Regeneration

[0025] (1) Silk fibroin is obtained in the same manner as in Example 1.

[0026] (2) Weigh 6g of silk fibroin with a balance, add it to 30ml of deionized water, swell at room temperature, stir and dissolve for 1-3 hours until the silk fibroin is completely dissolved, defoam for 1-3 hours until there are no bubbles in the solution, pour Into a flat mold of 5 x 5 cm, which was used in this embodiment to cast a film on a flat glass mold, volatilized for 2 days and then vacuum dried at 50°C for 7 hours to obtain a tissue isolation layer. At this time, scratch roughening with a probe to form staggered scratches with a depth of 1-10 microns on one side of the tissue isolation layer. Dissolve silk fibroin in deionized water to make a silk fibroin solution with a concentration of 30%, and then use electrospinning method to spray-spin on the above-mentioned scratched surface according to convention...

Embodiment 3

[0027] Embodiment 3: growth factor composite bioactive tissue regeneration film for bone tissue regeneration

[0028] (1) Silk fibroin is obtained in the same manner as in Example 1.

[0029] (2) Weigh 6g of silk fibroin with a balance, add it to 40ml of deionized water, swell at room temperature, stir and dissolve for 1-3 hours until the silk fibroin is completely dissolved, defoam for 1-3 hours until there are no bubbles in the solution, Pour into a 5 x 5 cm flat mold. In this embodiment, the flat glass mold is used to form a film by casting on a flat glass mold. After volatilization for 1 day, vacuum dry at 60° C. for 8 hours to obtain a tissue isolation layer. At this time, scratch roughening with a probe to form staggered scratches with a depth of 1-10 microns on one side of the tissue isolation layer.

[0030] (3) Add the freeze-dried powder of 0.5g bioactive factor recombinant human gene bone morphogenic protein to 100mL silk fibroin solution with a concentration of 30...

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Abstract

The invention discloses a bioactive tissue regeneration membrane and a preparing method thereof. The membrane is double compound layer. One layer is a tissue isolation layer which comprises silk fibroin, and the other layer is a tissue orientation layer which comprises silk fibroin bundles adhered on the tissue isolation layer or silk fibroin bundles mixed with bioactive factors. One side of the membrane is a smooth dense shape, and the other side is a loose porous structure. In the preparing method of the bioactive tissue regeneration membrane, the silk fibroin solution is swollen, stirred, deaerated and casted into membrane to obtain the tissue isolation layer. One side of the tissue isolation layer is scratched to be coarsened. Then, the silk fibroin solution or the silk fibroin solution mixed with the bioactive factors is coated or sprayed on the scratched layer by an electrostatic spinning method. After freezing and vacuum drying demoulding, the membrane is obtained. The membranehas the advantages of nontoxic and harmless property, perfect biocompatibility, good cell adhering growth property, abundant raw material and low cost and can substitute bio-medical materials of collagen and the like and widely applied to a plurality of clinical subjects.

Description

technical field [0001] The invention relates to a biomedical engineering material and a preparation method thereof, in particular to a three-dimensional tissue repair material capable of inducing tissue regeneration and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] Guided tissue regeneration is currently the best clinical method for tissue defect repair. Its principle is to use the physical barrier function of the biofilm to isolate the bone defect area from the surrounding tissue, creating a relatively closed tissue environment, so that the regeneration of bone tissue functions are maximized. Its application provides a new and effective way for the treatment of periodontal disease, insufficient bone mass in the implant area, repair of other bone defects, and fracture healing. [0003] Membrane materials can be mainly divided into absorbable and nonabsorbable membranes. ① Non-absorbable and non-degrada...

Claims

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Application Information

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IPC IPC(8): A61L27/48A61L27/54A61L27/58A61L27/22
CPCA61L31/16A61L31/005A61L2300/00
Inventor 张玉峰刘朝阳马兆成
Owner WUHAN UNIV
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