Method for preparing bone collagen polypeptide using animal bone

An animal bone and collagen technology, which is used in food preparation, animal protein processing, and application, etc., to achieve the effects of wide source of raw materials, low price, and easy control of reaction conditions.

Inactive Publication Date: 2009-07-08
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no document in the existing technology to use Protamex compound protease to enzymatically hydrolyze animal bones to prepare collagen polypeptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take the fresh pork bone and wash it, then break it into pieces, put the broken bone into a cooking container, add water of the same quality as the bone into the container, cook at 110°C and 143kPa for 50 minutes, and then remove the upper layer of fat. Drain and chop to obtain 3-4cm bone pieces, dry at 85°C for 7 hours, coarsely pulverize the bone pieces with a pulverizer, and then carry out ultrafine pulverization with an ultrafine pulverizer, and pass the ultrafine pulverized bone powder 160-mesh sieve; add water to the sieved bone meal to prepare a 10% mass concentration of bone slurry, put it in a container, sterilize it in a water bath at 85°C for 15 minutes, then cool it below 40°C, adjust the pH value to 7.5, and then add Enzymolysis substrate Protamex composite protease with 2‰ quality of bone powder (produced by Novozymes, Denmark, marked with an activity of 1.5AU / g (Anson unit / gram)), enzymolysis at a temperature of 55°C and a pH value of 7.5 for 10 hours , a...

Embodiment 2

[0017] Take the fresh pork bone and wash it, then break it first, put the broken bone into a cooking container, add water of the same quality as the bone into the container, cook at 115°C and 169kPa for 40 minutes, then remove the upper layer of fat, Drain and chop to obtain 3-4cm bone pieces, dry at 85°C for 7 hours, coarsely pulverize the bone pieces with a pulverizer, and then carry out ultrafine pulverization with an ultrafine pulverizer, and pass the ultrafine pulverized bone powder 160-mesh sieve; put the sieved bone meal into a 12% mass concentration bone slime solution, put it in a container, treat it in a water bath at 85°C for 15 minutes to sterilize, cool to below 40°C, adjust the pH value to 7.5, and then add Protamex composite protease with a bone powder mass of 2.2‰ as the enzymatic hydrolysis substrate was enzymolyzed for 12 hours at a temperature of 55°C and a pH value of 7.5. After the enzymolysis was completed, the enzyme was inactivated in a water bath at 85°...

Embodiment 3

[0020] Take the fresh pork bone and wash it, then break it into pieces, put the broken bone into a cooking container, add water of the same quality as the bone into the container, cook at 120°C and 198kPa for 30 minutes, then remove the upper layer of fat, Drain and chop to obtain 3-4cm bone pieces, dry at 85°C for 7 hours, coarsely pulverize the bone pieces with a pulverizer, and then carry out ultrafine pulverization with an ultrafine pulverizer, and pass the ultrafine pulverized bone powder 160-mesh sieve; put the sieved bone meal into a 15% mass concentration of bone slime solution, put it in a container, sterilize it in a water bath at 85°C for 15 minutes, cool it below 50°C, adjust the pH value to 7.5, and then add Protamex composite protease with a bone powder mass of 2.4‰ as the enzymolysis substrate was enzymolyzed for 14 hours at a temperature of 55°C and a pH value of 7.5. After the enzymolysis was completed, the enzyme was inactivated in a water bath at 85°C for 15 ...

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PUM

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Abstract

The invention discloses a method for preparing ossein polypeptide by using animal bones, comprising the following steps: the animal bones are washed and cooked in boiling water after being crushed primarily; after the fat on the surface of cooking liquor is removed, the bones are drained and dried; then coarse grinding and superfine grinding are carried out on the bones so as to obtain bone powder; after being added with water, the bone powder is prepared into bone cement liquor; after being sterilized at a high temperature, the bone cement liquor is cooled, then enzyme therein is resolved and killed by adding protamex; after filtration, the filter liquor is concentrated in vacuum and frozen and dried; finally, the ossein polypeptide is gained. The invention has the advantages of simple technique, wide raw material sources and low price. Additionally, the reaction condition is easy to control in the preparation process, thus greatly increasing the added use value of the animal bones.

Description

technical field [0001] The invention relates to a preparation method of a polypeptide, in particular to a preparation method of a collagen polypeptide. Background technique [0002] According to recent scientific research, the human body absorbs protein mainly in the form of peptides, and peptides are molecules composed of amino acids, which can be classified into polypeptides, oligopeptides, micropeptides, and small peptides. Peptides exist in living organisms, and all cells can synthesize polypeptides, but they are all regulated by polypeptides. Peptides have strong activity and diversity, and can use the physiological functions and biological activities of peptide nutrition to regulate the functions of various systems and cells in the human body. Physiological functions, activate relevant enzymes in the body. Therefore, peptides become an important substance for human health. [0003] At present, the technology of using biological enzymes to catalyze and synthesize biol...

Claims

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Application Information

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IPC IPC(8): A23J3/04A23J3/34A23L1/305A23L1/312A23L33/18
Inventor 李宗军李珂王远亮谢静肖荣
Owner HUNAN AGRICULTURAL UNIV
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