Biologic sensor for optical fast detection of surface cleanliness and its test method

Abstract

The invention provides an optical biosensor used for detecting surface cleanliness rapidly, and a testing method thereof. The sensor comprises a sampling stick, a detection cavity, a liquid storage tank and a reaction chamber. The sampling stick comprises a handle, a spacing ring, a sampling swab and a pointed casing; the liquid storage tank is provided with a plurality of uniform sealing pieces which are in fixed connection through screw threads, and an ATP extractant and a neutralization reagent used for neutralizing the remaining extractant are sealed therein; and the lateral wall of the reaction chamber allows light to pass through, and a sensitive reagent is fixed at the bottom part in advance. When in detection, firstly, the sampling swab of the sampling stick is used for daubing and sampling the surface of an object to be detected; then, the pointed casing is screwed downwards; the sampling stick is inserted into the sampling detection cavity; and with the consideration of the fluorescence strength during the detection of a sample by an optical device, the surface cleanliness of the object to be tested can be determined by comparing the light intensity value with the standard sanitary threshold value. The sensor provided by the invention has the advantages of simple operation, convenient use, rapid response and disposability, and can be widely applied to the object surface cleanliness real-time detection and the field hygiene supervision in industries, such as food processing, cosmetics production, medical service and the like.

Description

technical field [0001] The invention belongs to the application field of biosensing technology, and relates to a biosensor for optical rapid detection of surface cleanliness and a testing method thereof. Background technique [0002] At present, the food processing industry, the pharmaceutical industry and other fields related to human health are implementing the "Hazard Analysis and Critical Control Point System" (HACCP) and "Good Manufacturing Practice for Drugs" (GMP). The use of the HACCP system in the food industry has become very common. Its core is to replace the final product quality inspection by monitoring the surface hygiene status of each link in the production process. However, most enterprises and health supervision agencies still mainly evaluate the environmental health status based on the total number of bacteria on the surface of the object, and rely on the traditional or improved agar plate culture method for detection, which has the problems of slow detect...

AI Technical Summary

Problems solved by technology

In recent years, domestic patents CN1338003A and CN1804602A have been related to two kinds of pen-type devices used to detect the degree of surface pollution, but did not consider the use of samples in the detection process, the interference of extraction reagents on luminescent reagents, and the inactivation of liquid enzyme reagents, etc. question

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