Preparation of high-purity immunological activity yeast beta-1,3-dextran with immunological activity

A technology of immune activity and dextran, which is applied in the field of microorganisms, can solve the problems of unfavorable industrial production, product activity damage, high production cost, etc., and achieve the effects of retaining immune activity, lowering cholesterol, and being easy to operate

Active Publication Date: 2009-06-24
GUANGDONG FOOD IND INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there have been literature reports on the preparation of β-1,3-glucan and the preparation of β-1,3-glucan by yeast and beer waste yeast in China, such as acid method, alkali method, autolyzed-phenol Alkali method, etc. Although these methods have high yield and high purity, the activity of the product is destroyed, and the significance of dextran as a functional factor to enhan...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 100kg of beer yeast raw material, add 500kg of water, seal and pressurize, heat up to 110°C, pH is 7, continue to stir for 3 hours, break the yeast cell wall to dissolve dextran, cool down to 45°C under normal pressure, add 150g neutral Protease and 200 grams of mannanase, adjust the pH to 6, keep stirring at a constant temperature for 2.5 hours, use a hanging bag centrifuge, the centrifugal speed is 1000rpm, the filter bag is made of polyester material, 400 mesh pore size, centrifuge to collect the precipitate 1, add raw materials 5 times of water, after stirring evenly, use sodium hydroxide to adjust the pH of the aqueous solution to 7.5, react at 70°C for 1 hour, then neutralize the reaction solution with hydrochloric acid, collect the precipitate 2 by centrifugation with a hanging bag centrifuge, add 10 times of the raw material Add tap water to Precipitation 2, stir for 25 minutes, and centrifuge to collect Precipitation 3 with a hanging bag centrifuge, then ad...

Embodiment 2

[0028] Take 100kg of beer yeast raw material, add 800kg of water, seal and pressurize, heat up to 125°C, pH is 7, continue to stir for 5 hours, break the yeast cell wall to dissolve dextran, cool down to 50°C under normal pressure, add 100g neutral Protease and 150 grams of mannanase, adjust the pH to 6, keep stirring at a constant temperature for 3 hours, use a hanging bag centrifuge, the centrifugal speed is 1000rpm, the filter bag is made of polyester material, 400 mesh pore size, centrifugal collection to obtain precipitation 1, add raw material 5 After stirring evenly, use sodium hydroxide to adjust the pH of the aqueous solution to 8, react at 75°C for 2 hours, then neutralize the reaction solution with hydrochloric acid, collect the precipitate 2 by centrifugation with a hanging bag centrifuge, and add tap water 10 times the raw material Into precipitation 2, stirred for 25 minutes, centrifuged with a hanging bag centrifuge to obtain precipitation 3, added tap water 10 t...

Embodiment 3

[0030] Take 100kg of baker’s yeast raw material, add 1200kg of water, seal and pressurize, heat up to 130°C, pH is 7, continue to stir and react for 8 hours, break the yeast cell wall to dissolve dextran, cool down to 60°C under normal pressure, add 300g neutral Protease and 350 grams of mannanase, adjust the pH to 8, keep stirring at a constant temperature for 5 hours, use a hanging bag centrifuge, the centrifugal speed is 1000rpm, the filter bag is made of polyester material, 400 mesh pore size, centrifugal collection to obtain precipitation 1, add raw material 5 After stirring evenly, use potassium hydroxide to adjust the aqueous solution to pH 9, react at 80°C for 3 hours, then neutralize the reaction solution with sulfuric acid, collect the precipitate 2 by centrifugation with a hanging bag centrifuge, and add tap water 10 times the raw material Into the precipitation 2, stirred for 25 minutes, centrifuged with a hanging bag centrifuge to obtain the precipitate 3, added ta...

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PUM

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Abstract

The invention discloses a method for preparing a high-purification yeast immunocompetence beta-1, 3-hyskon. In the preparation method, yeast raw material, after the pretreatment of autolysis, experiences enzymolysis and the precipitation thereof is collected after centrifugal filtration; after alkali treatment, the precipitation is neutralized by adding acid, washed, dehydrated, dried and crashed, and then the high-purification yeast immunocompetence beta-1, 3-hyskon product is obtained. The preparation method of the invention has the advantages of moderate condition, simple technique, easy operation and high yield, conforming to the policy of national cleaner production. Therefore, the invention is an environment-friendly and industrially feasible technical route. The product obtained by using the preparation method of the invention is characterized by high purification and high activity and is capable of obviously strengthening human body immunity, inhibiting the growth of a tumor and lowering cholesterin, thus can be applied in the industries of food, medicine and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of glucan, in particular to a preparation method of high-purity yeast immune activity β-1,3-glucan. Background technique [0002] As early as the 1940s, Dr. Pillemer first discovered and reported that there is a substance in the yeast cell wall that can improve immunity. Further research found that the substance is a polysaccharide, yeast β-1, 3 glucan, and derived from This substance was isolated from baker's yeast; since the 1970s, more and more scientists have begun to study the physiological functions of yeast glucan, and now there is a consensus on its physiological mechanism, and it is believed that yeast glucan Sugar is a highly efficient immune function activator; on June 24, 2003, researchers from NIAID (National Institute of Allergy and Infectious Diseases) and AMRIID (Army Medical Research Institute of Infectious Diseases) in the...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/08C08B37/02
Inventor 钟细娥李春荣詹耀才高展炬
Owner GUANGDONG FOOD IND INST
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