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Purification method for separation of cozymase Q10

A purification method and coenzyme technology, applied in quinone separation/purification, organic chemistry, etc., can solve the problems of solvent recovery work difficulty, health and environmental impact, difficult separation, etc., achieve simple recovery work, increase the content of effective components, Effective ingredients enhance the effect

Inactive Publication Date: 2009-05-13
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One disadvantage is that in the chromatography step, the commonly used eluents are n-hexane and another hydrophobic organic solvent such as benzene, ether and petroleum ether. One of the characteristics of these solvents is that they are highly toxic. It is easy to affect people's health and environment; the other is that they are insoluble in water, and after miscibility with normal hexane, they are not easy to separate like this, which causes greater difficulties to the subsequent solvent recovery work. The present invention provides A way to change this

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] coenzyme Q 10 20L of fermentation broth with a titer of 1672μg / ml was filtered out to obtain 3.2kg of wet bacteria, added with 20L of absolute ethanol, stirred at 55°C for 30 minutes, and filtered to obtain 20.5L of a filtrate with a titer of 796μg / ml. Then soak once more in the same way to obtain 20.2 L of filtrate with a titer of 684 μg / ml. The soaking solution mixed twice has a potency of 740 μg / ml and a volume of 40.7 L. It is vacuum-concentrated to 12 L at 70° C., 2.5 L of water and 2.5 L of n-hexane are added, stirred for 10 minutes, and left to separate for 24 hours. The n-hexane layer was separated to obtain 2.5 L of column solution with a titer of 11608 μg / ml.

Embodiment 2

[0032] coenzyme Q 10 20L of fermentation broth with a titer of 1672μg / ml was filtered out to obtain 3.2kg of wet bacteria, added with 20L of absolute ethanol, stirred at 65°C for 30 minutes, and filtered to obtain 20.5L of a filtrate with a titer of 842μg / ml. Then soak again in the same way to obtain 20.2 L of filtrate with a titer of 726 μg / ml. The soaking solution mixed twice has a titer of 780 μg / ml and a volume of 40.7 L. It is vacuum concentrated to 12 L at 70° C., added with 2.5 L of water and 2.5 L of n-hexane, stirred for 10 minutes, and left to separate for 24 hours. The n-hexane layer was separated to obtain 2.5 L of column solution with a titer of 12108 μg / ml.

Embodiment 3

[0034] coenzyme Q 10 20L of fermentation broth with a titer of 1672μg / ml was filtered out to obtain 3.2kg of wet bacteria, added with 40L of absolute ethanol, stirred at 80°C for 30 minutes, and filtered to obtain 40.5L of filtrate with a titer of 752μg / ml. Concentrate in vacuo at 70°C to 12L, add 2.5L of water and 2.5L of n-hexane, stir for 10 minutes, and let the mixture stand for 24 hours. The n-hexane layer was separated to obtain 2.5 L of column solution with a titer of 11808 μg / ml.

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Abstract

The invention relates to a method for purifying coenzyme Q10. The method comprises the following steps: an upper column solution containing the coenzyme Q10 is subjected to chromatography through a silica gel column; and a mixed solvent of n-hexane and low-grade alcohol or a mixed solvent of n-hexane and low-grade ketone is subjected to elution to obtain eluent; and the eluent is decompressed, is condensed to be dried and is added with ethanol to obtain the coenzyme Q10. Through an elution mode, the method greatly reduces the difficult degree of the reclaiming operation of a subsequent solvent; and simultaneously, the content of effective ingredients of the coenzyme Q10 is improved.

Description

technical field [0001] The present invention relates to a kind of separation coenzyme Q 10 The purification method, specifically relates to a kind of coenzyme Q with silica gel chromatography 10 method of purification. Background technique [0002] coenzyme Q 10 Ubiquinone, also known as ubiquinone, is a fat-soluble quinone compound that is widely distributed in nature and mainly exists in yeast, plant leaves, seeds and cells of animal heart, liver and kidney. It is the cell's own natural antioxidant and a natural activator of cellular metabolism. And after years of research shows that coenzyme Q 10 It is a good adjuvant drug for heart disease, which can protect the heart in heart surgery without any side effects. Therefore, it has a very wide range of uses in clinical and health care, beauty and skin care, etc., and is more and more valued by people. [0003] Currently, coenzyme Q 10 There are three kinds of preparation methods: animal and plant tissue extraction met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/28C07C46/10
Inventor 卢伟良周苗
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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