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Astragalus polysaccharide calcium phosphate nano-paclitaxel composite injection, and preparation method

A technology of astragalus polysaccharides and paclitaxel, which is applied in drug combination, pharmaceutical formula, drug delivery, etc., can solve the problems of poor water solubility of paclitaxel, large side effects of excipients, and no specific targeting effect of tumor cells, so as to increase drug efficacy, Low cost of medicine, effect of improving anti-tumor properties

Inactive Publication Date: 2010-09-29
南京凯瑞尔纳米生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a compound injection of astragalus polysaccharide calcium phosphate nano paclitaxel and Its preparation method and application

Method used

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  • Astragalus polysaccharide calcium phosphate nano-paclitaxel composite injection, and preparation method
  • Astragalus polysaccharide calcium phosphate nano-paclitaxel composite injection, and preparation method
  • Astragalus polysaccharide calcium phosphate nano-paclitaxel composite injection, and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of astragalus polysaccharide calcium phosphate nano-paclitaxel compound injection by one-step method

[0033]Accurately weigh 4.0g of calcium chloride and dissolve it in 2000ml deionized water to make a calcium chloride solution; accurately weigh 4.0g of disodium hydrogen phosphate and dissolve it in 2000ml of deionized water to make a disodium hydrogen phosphate solution; Dissolve 2.0g sodium citrate in 400ml deionized water to make sodium citrate solution; accurately weigh 1g of astragalus polysaccharide and dissolve it in 100ml ultrapure water to make astragalus polysaccharide solution; precisely weigh 0.25g of semi-synthetic paclitaxel and dissolve it in 25ml Prepare paclitaxel solution in absolute ethanol.

[0034] Mix the above five solutions according to the volume ratio of 80:80:16:4:1, stir at room temperature for 48 hours, sonicate for 2 hours, centrifuge at 10,000 rpm for 15 minutes at 4°C, discard the supernatant, and dry the precipita...

Embodiment 2

[0037] Example 2: Preparation of astragalus polysaccharide calcium phosphate nano-paclitaxel compound injection by two-step method

[0038] Accurately weigh 4.0g of calcium chloride and dissolve it in 2000ml deionized water to make a calcium chloride solution; accurately weigh 4.0g of disodium hydrogen phosphate and dissolve it in 2000ml of deionized water to make a disodium hydrogen phosphate solution; accurately weigh citric acid Dissolve 2.0g of sodium in 400ml of deionized water to make sodium citrate solution; accurately weigh 1g of astragalus polysaccharide and dissolve it in 10ml of ultrapure water to obtain astragalus polysaccharide solution; accurately weigh 2.0g of naturally extracted paclitaxel and dissolve it in 200ml of ethanol solution Paclitaxel solution was obtained afterwards.

[0039] Calcium chloride solution, disodium hydrogen phosphate solution, sodium citrate solution and astragalus polysaccharide solution were mixed according to the volume ratio of 20:20...

Embodiment 3

[0043] Embodiment 3: Drug efficacy test

[0044] Divide 30 mice into blank group, control group and test group, with 10 mice in each group, weighing 18-22 g, half male and half male.

[0045] The blank group used 5% glucose solution for injection, the control group used commercially available paclitaxel injection, and the test group used the astragalus polysaccharide-paclitaxel composite injection with the inorganic salt nanoparticles obtained in Example 1 as the carrier.

[0046] The mouse transplanted liver cancer Heps experimental animal model was adopted, and healthy mice were inoculated with the mouse transplanted liver cancer Heps from Nanjing Cancer Institute as Heps model mice. After two days of feeding, the blank group was injected with 5% Glucose solution, control group tail vein injection commercially available paclitaxel injection, test group tail vein injection by the astragalus polysaccharide calcium phosphate nano-paclitaxel compound injection prepared by exampl...

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Abstract

The invention relates to astragalus polyose calcium phosphate nano taxol complex injection and a preparation method and the application thereof. 100 mL of injection comprises the following components: 0.01 to 1 g of astragalus polyose calcium phosphate nano to particles, 1.0 to 50 mg of taxol, 0 to 5.0 g of osmotic pressure regulator and appropriate amount of water. The pH value of the injection is 6.0 to 7.5. The preparation method comprises the following steps: blending the taxol with absolute ethyl alcohol to form taxol-absolute ethyl alcohol solution; blending the astragalus polyose with ultrapure water to form astragalus polyose solution; performing stirring and ultrasonic processing to the solution, rejecting the supernatant fluid after separation, and after depositing, drying and sterilizing, obtaining astragalus polyose calcium phosphate nano taxol complex granules used for injection, then adding the osmotic pressure regulator into the water for injection, and mixing with the astragalus polyose calcium phosphate nano taxol complex granules; after ultrasonic processing, astragalus polyose calcium phosphate nano taxol complex injection is formed. The complex injection can beused for the treatment or auxiliary treatment of tumor.

Description

technical field [0001] The invention relates to an astragalus polysaccharide calcium phosphate nano-paclitaxel composite injection and a preparation method thereof. In particular, the invention relates to an astragalus polysaccharide calcium phosphate nano-paclitaxel composite injection with astragalus polysaccharide-containing nanoparticles as a carrier and a preparation method thereof. technical background [0002] Nanotechnology is one of the most revolutionary technologies in the 21st century, and it is an interdisciplinary frontier science and technology. It is the crystallization of biology, medicine, materials science, chemistry, physics and other disciplines. As a space platform technology, nanotechnology has involved many disciplines and technical fields. Especially in the field of medicine, the development of pharmacy, bioengineering and medical equipment have made revolutionary breakthroughs, and have become one of the hot spots in medical, biomedical and pharma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/08A61K31/715A61K31/337A61K47/36A61P35/00
Inventor 张学智袁伟民冯鲁中河清赵冬梅
Owner 南京凯瑞尔纳米生物技术有限公司
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