Method for designing polypeptides for the nanofabrication of thin films, coatings and microcapsules by electrostatic layer-by-layer self assembly

A self-assembling, thin-film technology for applications in biomedicine and other fields that can address issues such as lack of biocompatibility, scaling costs, etc.

Active Publication Date: 2009-04-29
路易斯安那科技大学基金会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are difficulties in using proteins for this purpose
These difficulties include limited control over the multilayer structure (since protein surfaces are highly irregular and proteins generally do not adsorb to regularly structured surfaces), limitations on pH (due to pH-dependent protein solubility and structural stability) , lack of biocompatibility and cost of scale-up production when exogenous proteins are used (if the gene has not been cloned); unless the protein is identical in a readily available source (e.g., bovine), the protein will have to be obtained from the organism for which it is intended to be used, making the cost of large-scale production of the protein prohibitive

Method used

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  • Method for designing polypeptides for the nanofabrication of thin films, coatings and microcapsules by electrostatic layer-by-layer self assembly
  • Method for designing polypeptides for the nanofabrication of thin films, coatings and microcapsules by electrostatic layer-by-layer self assembly
  • Method for designing polypeptides for the nanofabrication of thin films, coatings and microcapsules by electrostatic layer-by-layer self assembly

Examples

Experimental program
Comparison scheme
Effect test

example 2

[0135] 2. Example 2—Experiments Involving Redesigned Cysteine-Containing Polypeptides

[0136] a. Peptide

[0137] The peptides used are:

[0138] Tyr Lys Cys Lys Gly Lys Val Lys Val Lys Cys Lys Gly LysVal Lys Val Lys Cys Lys Gly Lys Val Lys Val Lys Cys Lys Gly Lys Val Lys (SEQ ID NO: 5)

[0139] Tyr Glu Cys Glu Gly Glu Val Glu Val Glu Cys Glu Gly GluVal Glu Val Glu Cys Glu Gly Glu Val Glu Val Glu Cys GluGly Glu Val Glu (SEQ ID NO: 6)

[0140] Unlike the other peptides used in the experiments described here, these two were not designed using human genome information; they were designed for the sole purpose of evaluating the role of disulfide bond formation in peptide film stabilization.

[0141] b. to operate

[0142] AU experiments were performed at room temperature. All assembly experiments using QCMs were performed under the same conditions, except that air was used instead of nitrogen to dry the samples that were about to undergo oxidation. Assembly conditions were 10 m...

example 3

[0151] 3. Example 3—Experiments Involving Cysteine-Containing Designer Polypeptides

[0152] a.Material

[0153] The essential elements of the experiment were a quartz crystal microbalance apparatus; a silver-coated resonator (9 MHz resonant frequency); a negative 48-residue peptide (LN3) (SEQ ID NO: 4); and the following sequence termed "SP5" The positive 48-residue peptide:

[0154] Tyr Lys Gly Lys Lys Ser Cys His Gly Lys Gly Lys Lys Ser Cys His Gly Lys Gly Lys Lys Ser Cys His Gly Lys Gly Lys Lys Ser Cys His (SEQ ID NO: 7)

[0155] Like the other designed peptides discussed in section VII(E)(1) above, SP5 was designed using the method described in VII(B)(1) above to analyze the amino acid sequence of the human blood protein lactoferrin (gi|4505043) . ELBL buffer was 10 mM Tris, pH 7.4, 10 mM NaCl and 1 mM DTT. The lysis buffer was 10 mM KCl, pH 2. 2 mL peptide solutions of SP5 and LN3 were prepared by adding 4 mg of each peptide to 2 mL of the above buffer solution and ...

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Abstract

A method for designing polypeptides for the nanofabrication of thin films, coatings, and microcapsules by ELBL for applications in biomedicine and other fields.

Description

[0001] Cross References to Related Applications [0002] none. [0003] Statement Regarding Federally Sponsored Research or Development [0004] none. technical field [0005] The present invention relates to the fabrication of ultrathin multilayer films on suitable surfaces by electrostatic lamination self-assembly ("ELBL"). More specifically, the present invention relates to a method of designing polypeptides by ELBL for the Nanofabrication of thin films, coatings and microcapsules, and the application of this method in biomedicine and other fields. Background technique [0006] ELBL is an established technique in which ultrathin films are assembled by varying the adsorption of oppositely-charged polyelectrolytes. The process is based on the reversal of the charge on the surface of the film after each layer is deposited. figure 1 A schematic diagram of the general ELBL process is shown: thin films of oppositely charged polyions (cationic polyion 10 and anionic polyion ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C07K14/47
CPCC07K14/4723G01N33/68C07K14/001B82Y5/00C07K1/1133B82Y30/00A61P7/06
Inventor 唐纳德·T·海尼
Owner 路易斯安那科技大学基金会
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