Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for breeding high sulfurous acid-producing yeast and method for producing liquor using the yeast

A technology of alcoholic beverages and sulfurous acid, applied in the preparation of alcoholic beverages, beer fermentation methods, methods of using fungi, etc., can solve the problems of loss of fermentation characteristics, achieve the effect of inhibiting the generation of peculiar smell and improving the freshness retention period

Inactive Publication Date: 2009-04-29
KIRIN HOLDINGS KK +1
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even if mutants with target traits are obtained by these mutation methods, mutations can be randomly introduced at sites other than the target gene, thereby changing traits other than the target trait, for example, resulting in Loss of fermentative properties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for breeding high sulfurous acid-producing yeast and method for producing liquor using the yeast
  • Method for breeding high sulfurous acid-producing yeast and method for producing liquor using the yeast
  • Method for breeding high sulfurous acid-producing yeast and method for producing liquor using the yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] A DL-ethionine-resistant strain was obtained from the bottom fermentation yeast VTT-A69025 strain for production (obtained from the yeast bank of Kirin Brewery Company) by the following method. Specifically, a single colony of VTT-A69025 grown on a YPD agar medium supplemented with 2% agar was inoculated into 5 mL of a YPD liquid medium, followed by culturing at 20° C. for 3 days. Centrifuge (3000rpm×5 minutes) medium to obtain yeast cell fraction. Add 10 mL of sterile water to the cell part, shake the product well, and then centrifuge the product to obtain the yeast cell fraction again. Repeat this process to wash the yeast cells.

[0144] Suspend the washed yeast cell pellet in 5 mL sterile water. 150 μL of the suspension was coated with 10 mg / mL DL-ethionine, 0.17% Bacto Yeast NitrogenBase w / o Amino Acids and Ammonium Sulfate (Difco), 0.5% ammonium sulfate, 2% glucose, 0.1% lead nitrate and 2 % agar agar medium. Spread 2×10 agar medium in small Petri dishes 6 ye...

Embodiment 2

[0154] A DL-ethionine-resistant strain was obtained from the bottom fermentation yeast KBY011 strain for production by the following method. Specifically, a single colony of KBY011 grown on YPD agar medium supplemented with 2% agar was inoculated into 5 mL of YPD liquid medium, and then cultured at 20° C. for 3 days. Centrifuge (3000rpm×5 minutes) medium to obtain yeast cell fraction. After adding 10 mL of sterilized water to each fraction, it was shaken well and centrifuged again to obtain yeast cell fractions. Repeat this process to wash the yeast cells.

[0155] The obtained (washed) yeast cell pellet was suspended in 5 mL of sterile water, and then coated with 200 μL of the suspension supplemented with 10 mg / mL DL-ethionine, Bacto Yeast Nitrogen Base w / o Amino Acids and Ammonium Sulfate ( Difco), 0.5% ammonium sulfate, 2% glucose, 0.1% lead nitrate and agar medium of 2% agar. Use 2×10 6 Yeast cells were spread on agar medium in small Petri dishes for culture, and then ...

Embodiment 3

[0163] Subsequently, in order to cultivate compared with the parental strain, due to the increase in the metabolism of hydrogen sulfide from sulfate ion in the metabolic pathway of yeast hydrogen sulfide and sulfurous acid, and the increase in the synthesis of homocysteine ​​from O-acetyl homoserine The amount of metabolism in the acid pathway, and the strain that can increase the production of sulfurous acid without increasing the production of hydrogen sulfide, was tested using the genetic recombination method. The bottom fermentation yeast strain KBY011 for production was sporulated and meiotically divided. Using the isolated meiotic isolate B43, gene recombinants were obtained by the following method. Disruption of the YNL311C gene involved in the stabilization of the Met14 protein to increase the amount of metabolism in the pathway for the synthesis of hydrogen sulfide from sulfate ions. The HOM3 gene was then overexpressed to increase the amount of metabolism in the hom...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for breeding a yeast, with which the production of sulfurous acid which is important for maintaining freshness during brewing is increased and the production of hydrogen sulfide which is one of the causes for nasty odor is not increased based on analytical results of yeast metabolites and gene expression levels, and a method for producing a high quality liquor using the yeast. That is, the invention relates to a method for breeding a yeast in which the production amount of hydrogen sulfide is not increased compared with its parent cell line and the production amount of sulfurous acid is increased by increasing the metabolic flux in the synthetic pathway from sulfate ions to hydrogen sulfide and increasing the amount of synthesized homocysteine from O-acetylhomoserine in the hydrogen sulfide and sulfurous acid production pathways of yeast, and a method for producing a liquor using the yeast.

Description

technical field [0001] Based on the analysis results of yeast metabolites and gene expression levels, the present invention provides a method for cultivating yeast, which can increase the production of sulfurous acid without increasing the production of hydrogen sulfide that can cause off-flavors, which is essential for maintaining Freshness is very important. In addition, the present invention provides a method for producing high-quality alcoholic beverages using the yeast. Background technique [0002] Sulfurous acid is a compound with high antioxidant effect, which is widely used as an antioxidant in food, beverage, medicine and other fields. It is known that in beer brewing, a longer shelf life is achieved by increasing the concentration of sulfurous acid contained in the product. Sulfurous acid plays an important role in maintaining freshness. Therefore, if the content of sulfurous acid in products such as beer is increased, products with stable taste can be obtained...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/19C12C11/00C12G1/00C12N1/16C12N15/09C12R1/85
CPCC12N9/1241C12C12/006C12N9/1205C12N9/88C12N9/1029C12N9/1217C12N9/0051C12P3/00C12R1/85C12N9/0006C12N9/0008C12N1/185C12R2001/85
Inventor 善本裕人吉田聪港纪子曾我朋义
Owner KIRIN HOLDINGS KK
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com