Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production of novel bacterial exopolysaccharides by using Phyllobacterium sp.nov.921F bacterial strain

A technology of bacteria and strains, applied in the direction of bacteria, microorganisms, and methods based on microorganisms

Inactive Publication Date: 2009-04-29
OCEAN UNIV OF CHINA
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Phyllobacterium sp.nov.921F is capable of producing exopolysaccharides. According to the inventor's information and literature search, there is currently no research report on the exopolysaccharides produced by Phyllobacterium

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production of novel bacterial exopolysaccharides by using Phyllobacterium sp.nov.921F bacterial strain
  • Production of novel bacterial exopolysaccharides by using Phyllobacterium sp.nov.921F bacterial strain
  • Production of novel bacterial exopolysaccharides by using Phyllobacterium sp.nov.921F bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] 1. Isolation and purification of strains:

[0012] The acquisition of the Phyllobacterium sp.nov.921F bacterial strain of the present invention: collect fungal fruiting body samples from the remaining veins of Changbai Mountain, add to 25ml enrichment medium (sucrose 2g, NaNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 0.5g, NH 4 Cl0.5g, FeSO 4 0.01g, H 2 O 1000ml, pH7.2) Erlenmeyer flask, 30 ℃, 160r / min shake flask culture 3d. Then the enriched culture solution was properly diluted with sterile physiological saline, and 0.2ml was taken to coat the primary screening separation medium (sucrose 10g, NaNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 0.5g, NH 4 Cl 0.5g, FeSO 4 0.01g, agar 15g, H 2 O 1000ml, pH7.5) plate. Inverted culture at 30°C for 48 hours, a viscous milky white colony on the selected plate was obtained, and a bacterium capable of producing exopolysaccharide was obtained.

[0013] 2. Identification of strains:

[0014] The isolated strain was identified according...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a bacterium capable of producing bacterial exopolysaccharide, and provides a method for producing the bacterial exopolysaccharide by using the strain. The strain has the characteristics of simple nutritional requirement, easy culture, and high yield of the exopolysaccharide. In particular, a semisynthetic medium in the invention is used for to ferment the strain to obtain higher sugar yield. The strain is cultured in a gravy liquid medium containing 3 percent of sucrose for 72 hours, and the yield of a strain exopolysaccharide reaches 1.5 grams per 100 millimeters. The bacterial exopolysaccharide is white powder and is soluble in water, and the weight-average molecular weight thereof is 736 kD, wherein the apparent viscosity of 1.0 percent sugar solution at room temperature is 655 mps. A study on the property of the bacterial exopolysaccharide shows that the polysaccharide consists of glucose, galactose, and repetitive units formed by pyruvic acid substituents, and the mol ratio of the three components is 1 to 1 to 1. The polysaccharide is different from that produced from any industrial production strains in the past; at the same time, as the strain for producing the bacterial exopolysaccharide, the strain of the invention has the characteristics of high product yield, good stability, short production cycle and low cost, and can realize industrial application and production.

Description

technical field [0001] Biotechnology Background technique [0002] The currently reported strains capable of producing exopolysaccharides are the following: D-glucose, D-galactose, D-mannose, L-rhamnose and D- Acidic polysaccharide composed of glucuronic acid, exopolysaccharide composed of glucose and xylose produced by Chlamydomonas ulvaensis, and polysaccharide composed of D-mannose and arabinose of M.tuberculosis. Streptococci S. equi and S. zooepidernicus are able to produce hyaluronic acid consisting of glucuronic acid and glucosamine. Streptococcus thermophilus (S. Thermophilus) is capable of producing polysaccharides consisting of repeating units of D-galactose, D-glucose and N-acetylgalactose. Wild Xanthomonas Campestris can produce xanthan gum, which is composed of D-glucose, D-mannose, and D-glucuronic acid. There are also a few species that can produce homogeneous glycans, such as extracellular glucans produced by Leuconostoc sp. and Pediococcus sp.; extracellu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 江晓路王鹏管华诗
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com