Aroma type green plum stuffing as well as production method thereof and food with green plum stuffing
A production method and technology of Luzhou-flavor type, applied in the field of Luzhou-flavor greengage stuffing and its production and greengage stuffing food, can solve the problems of low added value and monotonous variety, and achieve the effect of strong fruity aroma and enhanced greengage flavor
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Embodiment 1
[0021] Embodiment 1 (flavor enhancing enzyme 1)
[0022] Aspergillus niger (Aspergillus niger) and Aspergillus oryzae (Aspergillus oryzae) were respectively activated by the strains, and were inserted into the fermentation medium at a 5% inoculation amount. The medium formula was: bran 6g / L, peptone 0.1g / L , Potassium dihydrogen phosphate 0.1g / L, salicin 0.1g / L, ammonium sulfate 0.1g / L, ferrous sulfate 0.01g / L. Mixed fermentation was carried out at 30°C and 300r / min for 3 days, the obtained fermentation broth was leached in a water bath at 40°C for 5 hours, the filtrate was filtered, centrifuged at 4000r / min for 30min at 2-4°C, and the supernatant was obtained as flavor enhancer. Fragrant Enzyme Liquid. The enzyme activity unit of β-glucosidase is defined as the amount of enzyme required to produce 1 μmol of glucose per minute by 1 mL of enzyme solution as one enzyme activity unit (U). It was determined that the β-glucosidase activity of the flavor-enhancing enzyme solution ...
Embodiment 2
[0023] Embodiment 2 (flavor enhancing enzyme 2)
[0024] Aspergillus niger (Aspergillus niger) and Aspergillus ficuum (Aspergillus ficuum) were respectively through bacterial classification activation, inserted into fermentation medium (bran 2g / L, peptone 0.5g / L, bisulphate iron 0.1g / L, ammonium sulfate 0.4g / L). Mixed fermentation was carried out at 40°C and 200r / min for 2 days, the obtained fermentation broth was extracted in a water bath at 60°C for 1 hour, the filtrate was filtered, and the filtrate was centrifuged at 6000r / min at 2-6°C for 15 minutes, and the supernatant was taken for flavor enhancement Enzyme solution. The enzyme activity unit of β-glucosidase is defined as one enzyme activity unit (U) for the amount of enzyme required to produce 1 μmol of glucose per minute in 1 mL of enzyme solution. It was determined that the β-glucosidase activity of the flavor-enhancing enzyme solution was 320 U / mL.
Embodiment 3
[0025] Embodiment 3 (flavor enhancing enzyme 3)
[0026] Aspergillus niger (Aspergillus niger), Aspergillus oryzae (Aspergillus oryzae) and Aspergillus ficuum (Aspergillus ficuum) were respectively through bacterial classification activation, divide into fermentation medium (bran 3g / L) by 5%, 3% and 2% inoculum amount , potassium dihydrogen phosphate 0.3g / L, peptone 0.3g / L, salicin 0.01g / L). Mixed fermentation was carried out at 20°C and 600r / min for 5 days, the obtained fermentation broth was leached in a water bath at 30°C for 6h, the filtrate was filtered, centrifuged at 3000r / min at 2-4°C for 60min, and the supernatant was obtained as flavor enhancer. Fragrant Enzyme Liquid. The enzyme activity unit of β-glucosidase is defined as one enzyme activity unit (U) for the amount of enzyme required to produce 1 μmol of glucose per minute in 1 mL of enzyme solution. It was determined that the β-glucosidase activity of the flavor-enhancing enzyme solution was 330 U / mL.
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