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Method for cultivating plant strain of distant hybrid lily

A cultivation method and lily technology are applied in the field of cultivation of distant lily hybrid plants, which can solve the problems of interspecific hybridization incompatibility, interspecific hybridization incompatibility, etc. Overcome the effects of far-hybrid incompatibility

Inactive Publication Date: 2009-04-22
GUIZHOU HORTICULTURAL INST (GUIZHOU HORTICULTURAL ENG TECH RES CENT) +2
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the incompatibility defect of lily interspecific hybridization in the prior art, and provide a method for cultivating distant source lily hybrid plants, which can better solve the incompatibility of interspecific hybridization question

Method used

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  • Method for cultivating plant strain of distant hybrid lily
  • Method for cultivating plant strain of distant hybrid lily

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Experimental program
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Embodiment approach

[0042] Materials: Purchase musk lily L-type "New Iron Cannon", OT-type lily "Yellow Storm" and O-type lily "Tiber" cut flower materials from the market, insert them into water, choose flower branches free from diseases and insect pests, and harvest 1-2 months before flowering. day buds.

[0043] Sterilization: On the ultra-clean workbench, select mature flower buds that have not yet opened, soak them in 75% alcohol for 1 minute, wash them with sterile water for 3 times, then disinfect them with 0.1% HgCl for 5 minutes, and wash them with sterile water for 3 minutes. Second-rate. The sterile filter paper was blotted dry, the petals were peeled off, and the ovary and anthers were cultured in MS medium + 0.02mg / l naphthaleneacetic acid medium respectively. The culture conditions were 25°C, light intensity 1500lux, and light time 12 hours. Sterilization is one of the keys. If the sterilization time is too long, the pollen and ovary will be easily damaged. If the sterilization tim...

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Abstract

The invention provides a method for cultivating hybrid plants of far-source lilies, which overcomes the problem that far-source hybrids are not easy to be affinitive in the prior art. The method performs in vitro culture to an ovary and an anther, adopts an unconventional pollination mode of cutting off the ovary for in vitro pollination, and then performs embryo culture so as to obtain a hybrid plant. Hybrid seedlings are obtained by use of the method, which proves that the method successfully overcomes the problem that the far-source hybrids are not affinitive, can well improve seed setting rate, and is worthy to be popularized and applied.

Description

technical field [0001] The invention relates to a hybrid plant cultivation method, in particular to a cultivation method of a distant lily hybrid plant. Background technique [0002] Lily is the general term for the plants of the genus Lillium of the Liliaceae (Liliaceae), a perennial herb. At present, there are about 115 species in the world, and 55 species and 18 varieties have been found in my country, accounting for half of the world's Lilium plants. At the beginning of the 20th century, foreign countries, centered on the Netherlands, Japan and the United States, carried out the breeding of lilies. They used the already excellent oriental, musk and Asian lilies to carry out intergroup and interspecific hybridization, and cultivated lilies with good commercial characteristics. New varieties, launching new hybrid varieties of OT and LA series. At present, the main cut lily varieties on the market are concentrated in five varieties including Asiatic hybrids, Oriental hybr...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00C12N5/04
Inventor 郑思乡刘妍刘飞虎王继华刘武林赵宁张朝君
Owner GUIZHOU HORTICULTURAL INST (GUIZHOU HORTICULTURAL ENG TECH RES CENT)
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