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Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes

A deer faceplate and gel filtration chromatography column technology, applied in the field of biomedicine, to achieve excellent hypoglycemic effect

Inactive Publication Date: 2009-04-08
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports on the structure of the active protein of the flower disc and the research work on the anti-diabetic active substances of velvet and flower disc. Therefore, this study is in line with the development direction of the modernization of traditional Chinese medicine in my country, and has important social significance and potential economic benefits in the field of basic research and applied research. benefit

Method used

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  • Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes
  • Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes
  • Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes

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Embodiment 1

[0026] Preparation of centrifuged supernatant: Take 5kg of deer discs, crush them, add 15,000ml of pre-cooled pH3.5 acetic acid solution, stir at 4°C for 30 minutes, and then -1 Refrigerate and centrifuge for 20 minutes under conditions, take the supernatant, and repeat the extraction of the precipitate once.

[0027] The above supernatants were combined, then filtered with Xinhua filter paper, the filtrate was collected, and the protein content was measured by the biuret method to be 11.194 mg / ml.

[0028] Superdex with FPLC TM G75 (purchased from Amfarmersia Biotechnology (China) Co., Ltd.) gel filtration chromatography column for purification, after the above supernatant was freeze-dried, 1ml of the sample (concentration: 50mg / ml) was loaded, eluted with distilled water, and collected Peak 2, its protein content measured by biuret method is 1.487mg / ml.

[0029] Take the RESOURSE of the FPLC on peak 2 above TM RPC3ml column, load 1ml (concentration is 1mg / ml), mobile pha...

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Abstract

The invention relates to the bio-medical field, particularly to a polypeptide separated from a deer antler base by using bioseparation engineering technology for preventing and treating diabetes. The relative molecular weight of the polypeptide is 7,127.6 daltons, the UV characteristic absorption peak is 205nm, lavender is shown on biurets reaction, and royal purple is shown on ninhydrin reaction. The polypeptide contains 16 kinds of amino acid. The polypeptide has good thermal stability and is stable in pH between 2 and 9. The polypeptide of the deer antler base is proved to have excellent effect of blood glucose reduction by the experiment of pharmacodynamics in vivo.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a polypeptide for preventing and treating diabetes from deer flower discs by using bioseparation engineering technology. Background technique [0002] Deer discs, also known as horn discs and pearl discs, are the antler feet that are left on the upper part of the horn base of sika deer or red deer when they saw the antlers. Folks have a long history of using antler discs to treat hyperplasia of mammary glands. Therefore, the chemical composition and pharmacological effects of the flower disc are similar to those of deer antler. The reported pharmacological effects of flower disc powder include: inhibiting mammary gland hyperplasia, anti-fatigue, improving the stress response caused by harmful factors, enhancing the body's hematopoietic function, anti-inflammatory, antibacterial and analgesic, promoting the phagocytosis of mouse macrophages and T Lymphocyte proliferation ability, inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/20C07K1/16A61K38/17A61P3/10
Inventor 吴梧桐黄凤杰钱璟吉静娴
Owner CHINA PHARM UNIV
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