Evanescent wave optical fiber biosensor and use thereof

Abstract

The invention relates to a biological detection device capable of being widely applied in fields such as molecular biology, biomedicine, food inspection, biological agents, environment monitoring and so on, in particular to an evanescent wave-based optical fiber biosensor capable of realizing quantitative and accurate detection to a biological target. The evanescent wave-based optical fiber biosensor comprises an optical detection system and an optical fiber bio-probe consisting of an optical fiber and capture biomolecules implanted on the surface of the optical fiber, wherein internal calibration ions of doped La elements or transition elements are contained in the body of the optical fiber, and the concentration of the internal calibration ions is between 500 and 4000ppm. The evanescent wave-based optical fiber biosensor realizes the quantitative detection of internal calibration reference in the internal calibration reference, simplifies the complex process that optical fiber biosensors for reference and detection are adopted to synchronously work in a semi-quantitative or quasi-quantitative biological detection technology of the prior art, and improves the stability of quantitative detection values and detection accuracy.

Description

technical field [0001] The invention relates to a biological detection device that can be widely used in the fields of molecular biology, biomedicine, food inspection, biological warfare agents and environmental monitoring, especially a biological detection device capable of detecting biological targets, such as bacteria, biological warfare agent molecules, viruses, Evanescent wave fiber optic biosensors for quantitative and accurate detection of deoxyribonucleic acid (DNA), peptides, antigens and antibodies and other biomolecules. Background technique [0002] At present, the widely used biological detection system based on optical fiber evanescent wave biosensor is to use the evanescent wave excitation generated during the transmission of light waves in the optical fiber in the form of total reflection to bind to the surface of the optical fiber probe with a bioaffinity reaction. Fluorescent dyes for biomolecules. A fiber optic bioprobe immobilized on the surface to captu...

AI Technical Summary

Problems solved by technology

However, there is a common problem in the optical fiber sensor system that uses fluorescence as the detection signal: there are differences between optical fibers, such as: small differences in the incident angle of the optical fiber, defects on the surface of the optical fiber, small fluctuations in the light source or immobilized antibodies The amount and other reasons will cause the distortion and large error of each measurement information

Therefore, two sets of detection systems are required, resulting in expensive, time-consuming and cumbersome operations, making this system unused in current evanescent wave optical fiber biosensors

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