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Extraction method of activity ingredient from katsura tree and uses thereof

An active ingredient, the technology of Cinnamon, applied in the field of biomedicine, can solve the problems that there is no public report on the anti-Bacillus cereus activity of Cinnamon, and achieve a strong inhibitory effect

Inactive Publication Date: 2009-03-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the literature, there is no public report that Lianxiangshu has anti-Bacillus cereus activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The branches of Lianxiangshu were washed with water and put into an oven, and baked at 60°C for 12 hours. 10 grams of dried branches were chopped, put into a conical flask, soaked in 100ml of 100% methanol, and extracted on a water bath at 45°C. The time for soaking and extracting for the first time was 2 hours, and the time for soaking and extracting for the second and third times were 4 hours and 8 hours respectively. The extracts from the three extractions were combined and concentrated at a temperature of 60° C. to obtain 0.521 g of extract. The extract was dissolved in methanol and prepared to a concentration of 10 mg / ml for activity determination. At the same time as a comparison, the results are shown in Table 1.

[0023] Table 1 The antibacterial effect of the extracts of Lianxiang twigs on Bacillus cereus

[0024]

dosage

1.5mg / disc dosage

1mg / disc dosage

0.5mg / disc dosage

0.1mg / disc Methanol

control blank

control ...

Embodiment 2

[0028] Basically the same as Example 1, the difference is that the material used is the leaves of Lianxiangshu. 10 grams of leaves were extracted to obtain 1.234 grams of extract, which was dissolved in methanol to prepare a concentration of 10 mg / ml for activity determination. At the same time as a comparison, the results are shown in Table 2.

[0029] Table 2 The antibacterial effect of the extract of the leaves of Lianxiang tree on Bacillus cereus

[0030]

dosage

1.5mg / disc dosage

1mg / disc dosage

0.5mg / disc dosage

0.1mg / disc Methanol

control blank

control Zone of inhibition

Diameter (cm) 1.2cm

0.9cm

0.8cm

-

-

-

[0031] - No zones of inhibition appear

[0032] Compared with the branch extract, the leaf extract has a larger amount, and its antibacterial activity is weaker than that of the branch extract. However, the antibacterial activity of the leaf extract also has obvious antibacterial effec...

Embodiment 3

[0034] It is basically the same as Example 1, except that the extracted extract is heated in a water bath at 80° C. for 1 hour, then dissolved in methanol, and prepared at a concentration of 10 mg / ml for activity. At the same time as a comparison, the results are shown in Table 3.

[0035] Table 3 The antibacterial effect of the extract of Lianxiang twigs on Bacillus cereus after high temperature treatment

[0036]

dosage

1.5mg / disc dosage

1mg / disc dosage

0.5mg / disc dosage

0.1mg / disc Methanol

control blank

control Zone of inhibition

Diameter (cm) 1.4cm

1.2cm

0.9cm

0.8cm -

-

[0037] - No zones of inhibition appear

[0038] The results showed that the samples after high temperature treatment had the same antibacterial activity as the samples without high temperature treatment, indicating that the active ingredient was stable to heat.

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PUM

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Abstract

The invention relates to an active ingredient extracted from katsura tree plant and the application thereof, belonging to the technical field of biological medicines. The active ingredient is obtained by the following steps: a. branches or leaves of katsura tree are cleaned by water and then placed into a baking box and baked for 12 hours at the temperature of 60 DEG C; b. the baked braches or leaves of katsura tree are cut into pieces and respectively placed into a triangular flask to be dipped in 100% methanol, and then extracted in a water bath of 45 DEG C, with dipping and extracting of the first time lasting 2 hours, dipping and extracting of the second time lasting 4 hours, and dipping and extracting of the third time lasting 8 hours; c. the extracting solutions of the three times are merged and then concentrated at the temperature of 60 DEG C to obtain extract; d. the obtained extract is dissolved by methanol to prepare the active ingredient with the concentration of 10 mg / ml; or after being heated in water bath at the temperature of 80 DEG C for 1 hour, the extract is dissolved by methanol to prepare the active ingredient with the concentration of 10 mg / ml. The extracted active ingredient in the invention can be used for preparing a preparation for inhibiting human pathogenetic bacteria Bacillus cereus, thus being suitable for being developed into new medical antibiotic.

Description

Technical field: [0001] The invention relates to an active ingredient extracted from a japonica plant and its application, which belongs to the technical field of biomedicine. Background technique: [0002] Bacillus cereus (Bacillus cereus) belongs to the Bacillus family, the genus Bacillus, is an aerobic, motile, Gram-positive bacteria that can produce spores. The bacterium is widely distributed in nature and exists in food such as soil, water, dust, starch products and dairy products. It can exist in the form of sporophytes in its life process, and has the characteristics of high temperature resistance, salt and alkali resistance, extrusion resistance, and little damage to it during feed processing. Studies have shown that the bacteria can produce a variety of toxic substances, such as enterotoxins, hemolytic toxins, etc. The food poisoning caused by it involves many types of food, and the potential risk of food poisoning is high. diseases have received attention. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P31/04
Inventor 张克勤刘芳芳李国红
Owner YUNNAN UNIV
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