Chemiluminescence immune analytic reagent kit for detecting tuberculosis antibody

Abstract

The invention relates to a reagent kit for detecting tuberculosis antibodies, and a preparation method thereof. The kit is composed of a chain avidin coated solid-phase vector, negative and positive controls, biotinylation tuberculosis antigens, alkaline phosphatase marker tuberculosis antigens, concentration washing solutions and chemiluminescent substrate solutions. The reagent kit adopts the double antigen sandwich reaction principle and the biotin-avidin system coated solid-phase vectors, the method not only has uniform and firm coating and greatly improves the antigen purity, but also can increase the antigen absorption, lower the cost and have the characteristics of high sensitivity, strong specificity and the like, can accurately detect the tubercubosis antibodies in various tuberculosis serums, and provide a reliable basis for clinical diagnosis and tuberculosis screening.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine. Specifically, the invention provides a chemiluminescence immunoassay kit for detecting tuberculosis antibodies and a preparation method thereof by combining chemiluminescence immunoassay with a biotin-avidin system. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Tuberculosis, also known as tuberculosis and "white plague", is an ancient infectious disease that can invade various organs of the human body. Tuberculosis is divided into 5 categories: primary pulmonary tuberculosis, hematogenously disseminated pulmonary tuberculosis, secondary pulmonary tuberculosis, tuberculous pleurisy and other extrapulmonary tuberculosis, etc. Tuberculosis is one of the most threatening infectious diseases to humans today, and it is also a single The most common cause of death caused by infectious agents has exceeded the total number of deaths...

AI Technical Summary

Problems solved by technology

[0003] The primary diagnostic techniques for tuberculosis mainly include: 1. Sputum smear examination: simple and easy to do, high accuracy, but low positive rate; 2. Sputum tuberculosis culture: High reliability, but time-consuming, expensive, and easily affected by medication, the application is limited; 3. Chest X-ray: accurate and fast, but easy to be confused with other lung diseases; the above diagnostic techniques generally have low sensitivity, poor specificity, and consumption Shortcomings such as length of time, so it has limited diagnostic value for tuberculosis

With the rapid development of molecular biology technology, highly sensitive methods are used to detect tuberculosis and its specific DNA fragments, such as PCR, biological probes and gene chips, etc., which have the advantages of simplicity, sensitivity and specificity in the detection of tuberculosis. However, corresponding testing equipment is required, the technical requirements are high, the cost is high, and it is difficult to promote

At present, ELISA, gold standard and other immunodiagnostic techniques for detecting tuberculosis antibodies in serum are simple and fast, do not require sophisticated instruments, and are easy to promote. However, the currently used diagnostic antigens have the disadvantages of high false positive rate and poor specificity. Antigenous and immunogenic diagnostic antigens, combined detection of several antigens can improve sensitivity without affecting specificity, and has higher sensitivity than single antigen method, which is of great significance to the diagnosis and methodological establishment of tuberculosis

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