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Kidney syndrome blooding diagnosis test paper strip, preparing method and detection reagent kit thereof

A technology for renal syndrome and hemorrhagic fever, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as application restrictions and complicated operations

Inactive Publication Date: 2009-03-04
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the commonly used laboratory detection methods for diagnosing hemorrhagic fever with renal syndrome include IgM antibody enzyme-linked immunosorbent assay (Mac ELISA), indirect immunofluorescence assay (IFA) and reverse transcription polymerase chain reaction (RT-PCR). Due to the complexity and the need for special instruments, its application in grassroots units is limited by conditions and speed

Method used

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  • Kidney syndrome blooding diagnosis test paper strip, preparing method and detection reagent kit thereof
  • Kidney syndrome blooding diagnosis test paper strip, preparing method and detection reagent kit thereof
  • Kidney syndrome blooding diagnosis test paper strip, preparing method and detection reagent kit thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0111] Embodiment 1 Preparation of test strips of the present invention

[0112] 1. Preparation of Immunocolloidal Gold

[0113] (1) Preparation of colloidal gold (40nm):

[0114] Measure 800ml of deionized water in a conical flask, add 8ml of 2% chloroauric acid solution to obtain a final concentration of 0.02% chloroauric acid aqueous solution, and accurately add 45ml of 1% trisodium citrate after boiling. Continue to heat and boil, the color of the solution turns from light yellow to black, and continue to boil until the solution gradually stabilizes to red. After heating for another 5 minutes, cool to room temperature, and restore to the original volume of 800 ml with deionized water.

[0115] (2) Determine the ratio of colloidal gold to the amount of goat anti-human μ chain antibody to be labeled by visual inspection

[0116] With 0.1mol / L K 2 CO 3 Solution Adjust the pH of the gold standard solution to 8.2, take a clean test tube and fill 1ml of colloidal gold, add ...

Embodiment 2

[0138] Instructions for using the test strip of the present invention for serum detection

[0139] 1. Detection principle

[0140] The diagnostic test strip of the present invention applies the principle of indirect capture immunochromatography, and the anti-HFRS IgM antibody in the patient's serum is combined with the colloidal gold-labeled goat anti-human μ chain antibody during the flow process, and migrates to the NC membrane detection line to interact with the Han The Tanvirus nucleocapsid protein antigen forms a complex to specifically bind and develop color.

[0141] 2. Reagent composition

[0142] HFRS diagnostic test strips and diluent 0.01% Tween-20 in PBS.

[0143] 3. Specimen collection

[0144] After the patient's blood is centrifuged to remove serum, it must be collected in clean, dry plastic tubes or glass containers that do not contain any preservatives. If it cannot be sent for inspection in time, it can be stored at 2-8°C for 24 hours. Long-term storage ...

Embodiment 3

[0156] Example 3 Detection kit for diagnosing hemorrhagic fever with renal syndrome

[0157] The detection kit for hemorrhagic fever with renal syndrome includes the diagnostic test strip for hemorrhagic fever with renal syndrome, diluent and instructions for use provided by the present invention. The diluent is a PBS solution of 0.01% Tween-20. Example 2.

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Abstract

The invention provides a diagnostic strip for hemorrhagic fever with renal syndrome, and a preparation method and a detection reagent kit thereof. The diagnostic strip is a colloidal gold immunochromatographic strip which is formed by that a sample absorption pad (1), a colloidal gold bonding pad (2), a pyroxylin film (5) and a water absorption pad (6) are sequentially stuck on a PVC soleplate (7) in a mutual lapping way. The diagnostic strip is characterized in that the colloidal gold bonding pad (2) is composed of glass-fiber membranes of goat anti-human Mu-chain immune gold complexes containing colloidal gold markers, a detection area (3) and a quality control area (4) are arranged on the pyroxylin film (5), the position of the detection area (3) is coated with hantavirus nucleocapsid protein antigens, and the position of the control area (4) is coated with rabbit anti-goat IgG antibodies. The diagnostic strip has good specificity, sensitivity and repeatability, can realize the early and rapid diagnosis of HFRS, and is easy to carry; and the operation is simple, the cost is low, and the response is fast. The invention is easy to be popularized in primary medical units.

Description

technical field [0001] The invention relates to a diagnosis technology of diseases, in particular to an early rapid diagnosis technology of hemorrhagic fever with renal syndrome (HFRS). Background technique [0002] Hemorrhagic fever with renal syndrome (HFRS) is a natural foci infectious disease caused by Hantavirus. my country is a high-incidence area of ​​HFRS. At present, cases have occurred in 31 provinces, municipalities, and autonomous regions in the mainland, and new epidemic areas continue to appear, and outbreaks are occasionally seen. The clinical manifestations of the disease are mainly fever, hemorrhage, and kidney damage. Non-specific symptoms such as fever, headache, and body aches in the early stage of the disease are difficult to distinguish from some common infectious diseases such as influenza and leptospirosis, so specificity Laboratory diagnosis is very important. [0003] Hantavirus (HV) is a genus of the family Bunyaviridae, and some virus strains of...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/544G01N33/532G01N33/52
Inventor 胡丽华王琳熊莉娟苏凤菊蔡开琳朱函坪罗端德揭盛华曾令兰肖菁
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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