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Mammary gland non-malignant tumor and malignant tumor blood serum special protein and uses thereof

A technology for serum and breast cancer, which is applied in the field of new tumor markers - early serum specific proteins of breast tumors, can solve the problems of distinguishing benign and malignant breast tumors, and achieve excellent results in sensitivity and specificity

Inactive Publication Date: 2009-01-28
福建省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the distinction between benign and malignant breast tumors. Therefore, it is of great significance to use mass spectrometry to analyze the serum of patients with benign and malignant breast tumors to obtain specific tumor markers.

Method used

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  • Mammary gland non-malignant tumor and malignant tumor blood serum special protein and uses thereof
  • Mammary gland non-malignant tumor and malignant tumor blood serum special protein and uses thereof
  • Mammary gland non-malignant tumor and malignant tumor blood serum special protein and uses thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Determination of serum specific proteins in the early stage of breast cancer

[0030] (1) Experimental samples

[0031] 1. Serum of breast cancer patients: 131 cases, all female, with a median age of 52 years old, diagnosed by clinicopathology and without surgery, radiotherapy and chemotherapy.

[0032] 2. Serum of patients with benign breast lesions: 91 cases, all female, with a median age of 46 years, diagnosed by clinicopathology and without surgery, radiotherapy and chemotherapy.

[0033] (2) Experimental materials

[0034] 1. Reagents and chips: urea, sodium acetate, acetonitrile, trifluoroacetic acid, DTT, and CHAPS were purchased from Sigma, USA; Sinapinic acid (SPA) and weak cation exchange chip (weak cation exchanger) CM10 were purchased from Ciphergen Biosytems, Inc Fremont , USA company.

[0035] 2. Protein chip reader: protein chip reader (PBSII-C type, Ciphergen Biosystems, Inc. Fremont, USA), according to the principle of laser desorption ion time-of-f...

Embodiment 2

[0069] A specific example of the classification model of serum protein spectrum related to benign and malignant breast cancer, which can assist in clinical judgment of the risk of breast cancer in patients with breast diseases

[0070] 1: Peng Moumou, female, 51 years old, medical record number 80346, the patient came to Fujian Cancer Hospital in March 2007 for 2 days due to the discovery of a tumor in the right breast. Space-occupying (inclined to right breast cancer) 2. Solid space-occupying in the upper outer quadrant of the left breast. The mammography showed that the local glands in the right breast were dense, and it was considered that the glands were incomplete. CEA, CA153, TPS were all normal. After admission, protein flight mass spectrometry was performed (see Figure 6 , 7), it can be seen that the protein peak intensity of the mass-to-nucleus ratio of 2766 is 14.9, the protein peak intensity of the mass-to-nucleus ratio of 9285 is 5.3, the protein peak intensity ...

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Abstract

The invention discloses a benign and malignant mammary tumour serum specific protein and application thereof, belonging to the field of biological detection technique. The invention uses an SELDI-TOF-MS technique, establishes the fingerprint of serum protein between the benign mammary tumour patient and breast carcinoma patient, carries out the variation analysis and searches new protein markers, thus dividing the benign and malignant mammary tumour. As the SELDI-TOF-MS technique is used by the invention, mass spectrometric detection, comparison and analysis are carried out on the serum of the peripheral blood of benign and malignant mammary tumour patients from the point of view of proteomics, thus establishing effective mammary tumour serum protein fingerprint which is used for the risk evaluation method used when the nasopharyngeal carcinoma is detected and distinguished outside the body. The method is a detection method with the sensitiveness and specificity better than that of the traditional method.

Description

technical field [0001] The invention relates to the technical field of biological detection, and in particular relates to a new tumor marker-a breast tumor early serum specific protein, which can detect and distinguish the occurrence of benign breast lesions and breast cancer in vitro. Background technique [0002] Breast cancer (mammary carcinoma) is a gene mutation in breast glandular epithelial cells under the action of various carcinogenic factors, resulting in uncontrolled cell proliferation. Breast cancer is the most common malignant tumor in humans and one of the main malignant tumors in women. The incidence of breast cancer varies greatly from country to country due to differences in geographical environment and living habits. Most countries in North America and Northern Europe have a high incidence of female breast cancer, some countries in South America and Southern Europe have a moderate incidence, and most of Asia, Latin America, and Africa have a low incidence....

Claims

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Application Information

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IPC IPC(8): G01N33/49G01N27/64
Inventor 应敏刚陈强叶韵斌陈慧菁陈夏郑弘宇吴凡
Owner 福建省肿瘤医院
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