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Protein chip for sironi virus detection and preparation method thereof

A technology of West Nile virus and protein chips, which is applied in the direction of measuring devices, biological tests, material inspection products, etc., to achieve the effect of accurate clinical diagnosis

Inactive Publication Date: 2012-12-19
SHENZHEN ACAD OF INSPECTION & QUARANTINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isolation and cultivation of West Nile virus needs to be carried out in professional experimental facilities, such as BSL-3 or BSL-4 laboratories, and pay attention to biosafety, but there are not enough laboratories in my country that meet this level of biosafety

Method used

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  • Protein chip for sironi virus detection and preparation method thereof
  • Protein chip for sironi virus detection and preparation method thereof
  • Protein chip for sironi virus detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, to the detection of human IgG

[0048] 1. Preparation of West Nile virus antibody multi-fragment protein chip

[0049] 1. Preparation of materials

[0050] CBS-G solution: containing carbonate buffer solution at 50% glycerol (mass percentage); The concentration of the carbonate buffer solution is 0.05mol / L, and the pH is 9.6;

[0051] Quality control control (horseradish peroxidase-labeled anti-human IgG antibody): purchased from Shanghai Biyuntian Biotechnology Co., Ltd. (A0201);

[0052] West Nile virus E protein: purchased from the United States abcam company (ab48960);

[0053] West Nile virus prM protein: purchased from American abcam company (ab39905);

[0054] West Nile virus M protein: purchased from the United States abcam company (ab39904);

[0055] Blocking solution: PBS solution containing 0.5% BSA (mass percentage) and 0.05% Tween 20 (mass percentage); the concentration of the PBS solution is 0.01 mol / L, and the pH is 7.4.

[0056] Glass ...

Embodiment 2

[0084] Embodiment 2, to the detection of human IgM

[0085] 1. Preparation of West Nile virus antibody multi-fragment protein chip

[0086] 1. Preparation of materials

[0087] CBS-G solution: containing carbonate buffer solution at 50% glycerol (mass percentage); The concentration of the carbonate buffer solution is 0.05mol / L, and the pH is 9.6;

[0088] Quality control control (anti-human IgM antibody labeled with horseradish peroxidase): purchased from Guangzhou Huatuo Biotechnology Co., Ltd. (HT21007);

[0089] West Nile virus E protein: purchased from the United States abcam company (ab48960);

[0090] West Nile virus prM protein: purchased from American abcam company (ab39905);

[0091] West Nile virus M protein: purchased from the United States abcam company (ab39904);

[0092]Blocking solution: PBS solution containing 0.5% BSA (mass percentage) and 0.05% Tween 20 (mass percentage); the concentration of the PBS solution is 0.01 mol / L, and the pH is 7.4.

[0093] Gl...

Embodiment 3

[0118] Embodiment 3, to the detection of human IgG

[0119] 1. Preparation of West Nile virus antibody multi-fragment protein chip

[0120] 1. Preparation of materials

[0121] CBS-G solution: containing carbonate buffer solution at 30% glycerol (mass percentage); The concentration of the carbonate buffer solution is 0.01mol / L, and the pH is 9.0;

[0122] Quality control control (horseradish peroxidase-labeled anti-human IgG antibody): purchased from Shanghai Biyuntian Biotechnology Co., Ltd. (A0201);

[0123] West Nile virus E protein: purchased from the United States abcam company (ab48960);

[0124] West Nile virus prM protein: purchased from American abcam company (ab39905);

[0125] West Nile virus M protein: purchased from the United States abcam company (ab39904);

[0126] Blocking solution: PBS solution containing 0.01% BSA (mass percentage) and 0.01% Tween 20 (mass percentage); the concentration of the PBS solution is 0.001 mol / L, and the pH is 6.5.

[0127] Glas...

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Abstract

The invention discloses a protein chip for detecting Silone virus and a process for preparation. The protein chip for detecting Silone virus is fixed with a biological chip of at least a Silone virus proteantigen on the chip base of the biological chip. The invention has the advantages of saving time, high effective and high flux parallel detection, detecting results not only can be qualitatively observed by naked eyes, but also can be qualitatively detected by a scanner, the results can be permanently conserved, the protein chip can not be compared by a traditional detection method, which is the crystallization of the existing biochip technology, the immunological technique and the chemical technology. On one aspect, the protein chip can more accurately do clinical diagnosis for Silone virus, on the other aspect, the protein chip can visually know the function which is played by each protein component of Silone virus in the aspect of immune response, which can provide a theoretical basis for researching subunit vaccine, and can provide technical support for background survey of epidemiology, and can create a precondition for preventing and curing illness.

Description

technical field [0001] The invention relates to a protein chip for detecting West Nile virus and a preparation method thereof. Background technique [0002] West Nile virus (WNV) was first isolated from the serum of patients with fever in the West Nile region of Uganda, Africa in 1937, hence the name. The virus can cause fever, known as West Nile fever. [0003] West Nile virus belongs to group B arboviruses belonging to the family Flaviviridae, and its genome is a single positive-sense RNA consisting of 11029 nucleotides. The main vertebrate hosts of West Nile virus are birds. Virus-infected birds and sick birds are the main sources of infection and storage hosts. Mosquitoes, especially Culex mosquitoes, are the main vectors. After the bird is infected with the virus, it usually does not appear clinical symptoms immediately, but has a long-lasting and high-titer viremia period. At this time, after being bitten by a mosquito, it can be transmitted by infecting the mosquito....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/68
Inventor 顾大勇徐云庆刘春晓赵纯中史蕾朱玉兰林连成
Owner SHENZHEN ACAD OF INSPECTION & QUARANTINE
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