Use of reporter plasmid for biological detection of radioactive ray dose

Abstract

The invention relates to an application of a reporter plasmid for biological detection of radiation dose. The application comprises the following steps that a shuttle vector between radiation resistant bacteria and coliform bacteria is taken as a foundation so as to form a reporter plasmid of the shuttle vector which has luciferase gene activity and is positioned between the radiation resistant bacteria and the coliform bacteria; the reporter plasmid is transformed into a wild type R1 of radiation resistant bacteria to obtain transformant radiation resistant bacteria which are irradiated by gamma ray; and according to the luminous quantity of a character transformant, luminous quantities after irradiation by gamma rays in different doses are measured. The reporter plasmid can be used for biological detection of high radiation dose and low radiation dose, and can be widely used in the detection of nuclear facilities such as a nuclear power station, radiation irradiation facilities in ahospital, and the like, and other places with radioactive sources; moreover, the reporter plasmid can also be used in contamination detection for radioactive substances in an environment, and can induce a plurality of DNA damaging agents as a bioinductor; meanwhile, the reporter plasmid has a luciferase gene, and plays a positive role in realtime monitoring of aspects of procreation, distributionand environmental influence of a recombined transformant, and so on.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene plasmid and its construction method and application, that is, a reporter plasmid for biological detection of radiation dose and its construction method and application. Background technique [0002] The health hazards of ionizing radiation and radiation safety assessment, the classification, diagnosis and treatment of acute and chronic radiation injuries under various conditions all need to understand and determine the exposure dose. The biological detection of radiation dose is the use of biological materials such as tissues, cells, DNA, proteins, etc. after ionizing radiation, which has a certain amount-effect relationship with the radiation dose. A class of biomarkers and analytical methods used to measure radiation doses through biological changes and analysis. The greatest advantage of using living organisms for dosimetry compared to physical detection is directness and fa...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for biological detection of radiation dose. Commonly used are: the relationship between the comet tail formed by DNA damage after radiation exposure and the comet core expressed by undamaged cells to study single-cell gel electrophoresis for predicting radiation dose (Comet test) method (Berwick and Vineins, 2000); there is also a chromosomal aberration analysis method (Ballarini and Ottolenghi, 2003; Duran et al., 2002; Camparoto et al., 2003), but the common disadvantages of these methods are complex operation, high detection cost, more human factors in damage observation, and difficult to quantify accurately, etc. Disadvantages: Although the use of auxiliary means such as computers can properly improve the accuracy of its measurement, these methods can only detect and measure low-dose radiation, and there is no method for biological detection and measurement of high-dose radiation