Method for separating human serum albumin

Abstract

The invention relates to a protein separation and purification process method in the biological product technology, in particular to a human serum albumin separation method, comprising protein separation and purification process. The method is characterized in that the method takes plasma as raw material; first of all, composition I, composition II and composition III are respectively precipitated; then the composition I, II and III are separated together; finally compositions IV-1 and IV-4 are precipitated respectively and separated together; the solid-liquid separation uses a pressure filtration technology, with the fluid intake pressure no more than 0.2MPa. The method of the invention adopts the pressure filtration technology in the separation process, adjusts and controls the ethanol albumin concentration, temperature, pH value and other parameters in the albumin separation, and overcomes the disadvantages that the existing technology which produces human albumin through low-temperature ethanol separation has lower albumen composition yield and the unstable substances (such as lipoprotein) in the albumin composition are not completely removed. Compared with the low-temperature ethanol method, the method of the invention has high yield of albumin (not less than 2.9g / 100ml) and good product quality.

Description

technical field [0001] The invention relates to a protein separation and purification process in biological product technology, in particular to a separation method of human blood albumin. Background technique [0002] Human albumin is the most abundant macromolecular protein in human blood. Since Cohn et al. created the low-temperature ethanol human plasma protein separation technology in the 1940s, the industrialized production of plasma protein has made great progress. [0003] The current low-temperature ethanol separation method to produce human serum albumin has the disadvantages of low yield of protein components and incomplete removal of unstable substances (such as lipoproteins) in the albumin component. There are mainly two reasons for the above-mentioned defects. One is that the selection of process parameters such as ethanol concentration, temperature, and pH value is unreasonable, and the other is that the separation method is unscientific. At present, most of t...

AI Technical Summary

Problems solved by technology

[0003] The current low-temperature ethanol separation method to produce human serum albumin has the disadvantages of low yield of protein components and incomplete removal of unstable substances (such as lipoproteins) in the albumin component.

There are mainly two reasons for the above-mentioned defects. One is that the selection of process parameters such as ethanol concentration, temperature, and pH value is unreasonable, and the other is that the separation method is unscientific. At present, most of them use centrifugation. When the temperature rises, the miscellaneous proteins are dissolved but not removed, and the unstable substances in the albumin component cannot be removed accurately.