Tissue engineering trachea implant and construction method and use

Abstract

The invention provides a trachea implant in tissue engineering. Chondroblast is combined with degradable biomaterial and then cultured and constructed jointly in vitro and in vivo, and has the blood supply connection with cultured tissues in vivo. The trachea implant in tissue engineering of the invention can effectively repair the segmental defect of the trachea. The invention further provides a construction method and a use method thereof.

Description

technical field [0001] The present invention relates to the fields of medicine and biomedical engineering, and more particularly relates to a tissue engineered trachea graft and its construction method and application. Background technique [0002] Tracheal defects caused by various reasons (congenital malformation, trauma, tumor, etc.) are common and life-threatening. The mortality rate of long-segment tracheal stenosis can be as high as 77%, and the mortality rate of long-segment tracheal atresia is basically 100%. Long-segment tracheal defects cannot be anastomosed directly, so tracheal substitutes are required for repair. Currently, there are three types of tracheal transplantation substitutes commonly used clinically, but each has its own shortcomings, as shown in Table 1. Therefore, there is still a lack of ideal tracheal substitutes, and the repair of long-segment tracheal defects has thus become a clinical problem. [0003] The development of tissue engineering pro...

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Problems solved by technology

[0008] 1) The cartilage support strength of the tissue engineered trachea constructed purely in vitro is insufficient, making it difficult to maintain the ventilation function;

[0009] 2) Although the simple construction in vivo is helpful to improve the mechanical strength of cartilage, direct placement in the body will cause a strong inflammatory response due to the acidic degradation products of commonly used PGA materials in animals, and it is difficult to form a good tissue engineering trachea;

[0010] 3) Some studies choose to construct in nude mice first to reduce the above-mentioned inflammatory response, but the mouse-derived cells are mixed into the tissue-engineered trachea, and then transplanted into experimental animals with sound immunity will cause heterogeneous immune rejection;

[0011] 4) In the previous studies, the free transplantation of tissue engineered trachea was used. It takes time to establish blood supply connection with the surrounding tissues after transplantation, and the tracheal bed is not a region with rich blood supply, so free transplantation is not conducive to the survival and development of tissue engineered trachea. Integration with normal tissues;

[0013] 6) The constructed tissue engineered trachea has no ciliated epithelium. If the adjacent ciliated epithelium cannot be covered in time after surgery, airway secretions cannot be effectively removed

These deficiencies in previous experimental constructions ultimately hindered the rapid integration of tissue engineered trachea transplantation, failing to reflect the superiority of tissue engineering technology and resulting in failure

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