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Distant hybridization method of Oncorhynchus masou and Oncorhynchus mykiss

A technology of distant hybridization and mountain female trout, which is applied in medical science, climate change adaptation, animal childbirth, etc., can solve the problems of poor stress resistance, low seedling emergence rate, and high mortality of broodstock, and achieve strong stress resistance and survival rate. High, maneuverable effect

Inactive Publication Date: 2008-12-17
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of low seedling emergence rate, poor stress resistance and high mortality of broodstock after breeding under the condition of artificial culture, and to provide a method for distant hybridization of female trout and rainbow trout

Method used

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  • Distant hybridization method of Oncorhynchus masou and Oncorhynchus mykiss

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specific Embodiment approach 1

[0007] Specific embodiment one: the method for remote hybridization of female trout and rainbow trout in this embodiment is realized by the following steps: one, rainbow trout and female trout are domesticated and cultivated respectively, so that the female rainbow trout and the male trout reach synchronous maturity ; 2. Squeeze out the eggs of rainbow trout females and put them into the insemination basin, then squeeze the semen of the male trout into the insemination basin equipped with eggs, stir quickly and evenly with soft and clean feathers, so that the sperm and eggs can fully contact, and then add them to hatch Put it in water for 1-2 minutes, and then wash off the excess semen and damaged egg skin with water to obtain fertilized eggs; 3. Put the fertilized eggs into the hatching water and let them stand for 30-40 minutes until they fully absorb water and swell, and the egg membranes harden and become rich. The temperature of the water used for hatching is 4-13°C. Durin...

specific Embodiment approach 2

[0035] Specific embodiment two: this embodiment is different from specific embodiment one: the steps of the method for domestication and cultivation of female trout in step 1 are as follows: from August, the male and female fish of female trout will be fed separately, and the male trout will be removed from the male trout. In addition to receiving normal sunlight, artificial light should be increased for 3 to 5 hours every night, and the female mountain trout should be covered to control the sunshine time at 8 to 10 hours per day. To cultivate. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0036] Embodiment 3: This embodiment is different from Embodiment 2 in that: in step 1, female trout are continuously domesticated and cultivated for 3 to 6 generations. Others are the same as in the second embodiment.

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Abstract

A distant hybridization method of Oncorhynchus masou and Oncorhynchus mykiss relates to a distant hybridization method of cold water fishes. The invention resolves the problems of Oncorhynchus masou of nonsynchronous male and female maturing, low rate of emergence, bad adversity resistance, high mortality rate of ripe fishes after propagation, and the like, under the conditions of artificial cultivation. The steps of the inventive method are as follows: firstly, cultivations are respectively carried out, so that progenitive parents can be synchronously matured; secondly, artificial fertilization is carried out; thirdly, breeding is carried out. The method of the invention has stronger operability and good suitability, thereby avoiding the limitations of a hormone-induced method. The first filial generation of coenospecies cultivated by the invention has fast growing, tender meat, strong adversity resistance and high survival rate after cultivation, thereby having lower cultivation cost; compared with self cross of Oncorhynchus masou, the growth rate is increased by 10-15%, the survival rate is increased by 30%, the fertilization rate is above 90%, the hatchability rate is above 80%, after propagation, the mortality rate is below 8.7%, and the indexes of fleshcolor, tenderness, pH value, water loss rate and water holding capacity are all in accordance with the requirements of excellent-quality cold water fishes.

Description

technical field [0001] The invention relates to a method for distant hybridization of cold-water fishes, in particular to a method for utilizing distant hybridization of trout and rainbow trout. Background technique [0002] Mountain female trout (Oncorhynchus masou masou) belongs to the family Salmonidae and the genus Salmon. It is native to Japan and was domesticated as a domestic fish in the 1950s. Our country was introduced by the Heilongjiang Fisheries Research Institute in 1996. The fish has a graceful body, elegant color, delicate meat, and fresh taste. It is deeply cherished by the people and has a high market price. However, the female trout is a small fish, and the sexually mature individual is small (generally only about 300g), and under the condition of pond culture, the male and female trout mature asynchronously, and the male fish matures about half a month earlier than the female fish, resulting in eggs The quality of the sperm used during insemination is rel...

Claims

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Application Information

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IPC IPC(8): A01K61/00A61D19/02
CPCY02A40/81
Inventor 张玉勇白庆利牟振波石连玉
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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