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Colophony based functional polymer immobilization diastase and preparation method thereof

A technology of functional polymer and amylase, which is applied in the direction of fixing on/in the organic carrier, to achieve the effect of easy-to-obtain raw materials, low price and wide application

Active Publication Date: 2008-12-10
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Nelson and Griffin used activated carbon to adsorb sucrase in 1916, and successfully used immobilized acyl amino acid hydrolase to produce L-amino acids in 1969, immobilized enzymes have become more and more extensive and in-depth due to their good physical and chemical properties. There are four methods, adsorption method, embedding method, covalent bonding method and cross-linking method, maleic rosin ethylene glycol acrylate-acrylic acid copolymer and maleic rosin ethylene glycol acrylate-acrylic acid copolymer immobilized starch The preparation method of enzyme has not been reported in the literature so far

Method used

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  • Colophony based functional polymer immobilization diastase and preparation method thereof
  • Colophony based functional polymer immobilization diastase and preparation method thereof
  • Colophony based functional polymer immobilization diastase and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Weigh 15g of maleic rosin ethylene glycol acrylate-acrylic acid copolymer, soak the acrylic resin with cyclohexane for 24h, and filter. Place it in a three-necked bottle and add 30ml of cyclohexane. Slowly add 15ml of SOCl to the three-necked flask with a constant pressure funnel 2 , Stir, about 1h drop. Heating to raise the temperature, the temperature is controlled at 75-80°C, and refluxed for 7 hours. After the reaction is over, change the distillation device to steam out excess SOCl 2 and cyclohexane. Finally, the acylated resin was washed 3 times with 20 ml of cyclohexane each time. Maleic rosin ethylene glycol acrylate-acrylic acid copolymer acylated resin was obtained.

[0053] Weigh 10 g of maleic rosin ethylene glycol acrylate-acrylic acid copolymer acylation resin, add 40 ml of cyclohexane and 50 ml of 3% amylase solution. Stir the solution with magnetic stirring for about 10 h, and operate under dry conditions during the whole process. Wash and filter ...

Embodiment 2

[0055] Weigh 40g of maleic rosin ethylene glycol acrylate-acrylic acid copolymer, soak the acrylic resin with cyclohexane for about 1 day, and filter. Place it in a three-necked bottle and add 50ml of cyclohexane. Slowly add 25ml of SOCl to the three-necked flask with a constant pressure funnel 2 , stirring, about 1.5h dripping. Heating to raise the temperature, the temperature is controlled at 75-80°C, and refluxed for 7 hours. After the reaction is over, change the distillation device to steam out excess SOCl 2 and cyclohexane. Finally, the acylated resin was washed 3 times with 30 ml of cyclohexane each time. Maleic rosin ethylene glycol acrylate-acrylic acid copolymer acylated resin was obtained.

[0056] Weigh 20g of maleic rosin ethylene glycol acrylate-acrylic acid copolymer acylation resin, add 40ml of cyclohexane and 60ml of 4% amylase solution. Stir the solution with magnetic stirring for about 12 hours, and operate under dry conditions during the whole process...

Embodiment 3

[0058] Weigh 60g of maleic rosin ethylene glycol acrylate-acrylic acid copolymer, soak the acrylic resin with cyclohexane for about 1 day, and filter. Place it in a three-necked bottle and add 50ml of cyclohexane. Slowly add 35ml of SOCl to the three-necked flask with a constant pressure funnel 2 , Stir, about 2h drop. Heating to raise the temperature, the temperature is controlled at 75-80°C, and refluxed for 8h. After the reaction is over, change the distillation device to steam out excess SOCl 2 and cyclohexane. Finally, the acylated resin was washed 3 times with 50 ml of cyclohexane each time. Maleic rosin ethylene glycol acrylate-acrylic acid copolymer acylated resin was obtained.

[0059] Weigh 30g of maleic rosin ethylene glycol acrylate-acrylic acid copolymer acylation resin, add 50ml of cyclohexane and 70ml of 5% amylase aqueous solution. Stir the solution with magnetic stirring for about 5 hours, and operate under dry conditions throughout the process. Wash an...

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Abstract

The invention discloses a rosinyl functional polymer immobilized amylase and a preparation method thereof. The amylase is a maleated rosin glycol acrylic ester-acrylic acid copolymer immobilized amylase prepared from a maleated rosin glycol acrylic ester-acrylic acid copolymer and an amylase. The activity is between 20 and 30mg.g<-1>.5min<-1>, the residual activity after being repeated use for thirteen times is not lower than 6 mg.g<-1>.5min<-1>, and the relatively retained activity is not lower than 20 percent. The invention also discloses the preparation method for the immobilized amylase. The immobilized amylase can be used for catalyzing the starch to be hydrolyzed to generate dextrin and reducing sugar, and has wide application in the food, light industry and medicine fields.

Description

technical field [0001] The invention relates to a rosin-based functional macromolecule immobilized amylase and a preparation method thereof, more specifically the invention relates to a maleic rosin ethylene glycol acrylate-acrylic acid copolymer immobilized amylase and a preparation method thereof. Background technique [0002] As a biocatalyst, enzymes have the advantages of high catalytic efficiency, strong specificity, mild reaction conditions, biodegradability and no environmental pollution; however, enzymes are very sensitive to environmental influences, and various chemical, physical and biological factors (including Temperature, pressure, organic solvents, ionic strength, pH, etc.) can all cause enzymes to lose their biological activity. Therefore, the research on immobilized enzymes has been paid attention to. Immobilized enzymes have catalytic properties, have the advantages of being recyclable and reusable, and the production process can be continuous and automate...

Claims

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Application Information

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IPC IPC(8): C12N11/08
Inventor 雷福厚卢建芳李鹏飞蓝虹云姚兴东刘祖广
Owner GUANGXI UNIV FOR NATITIES
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