Novel snake venom polypeptide, preparation and application thereof

A technology of snake venom polypeptide and pit viper, applied in the field of biochemistry, can solve the problem of less anti-tumor and achieve good anti-tumor effect.

Inactive Publication Date: 2008-12-03
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

According to literature reports, domestic scholars only isolated some nerve growth factors and anti-platelet aggregation polypeptides from Agkistrodon venom, and foreign scholars only isolated a neurotoxin with cytotoxicity from Agkistrodon venom, and Agkistrodon halys venom The high-purity anti-tumor active ingredients in snake venom are still relatively few

Method used

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  • Novel snake venom polypeptide, preparation and application thereof
  • Novel snake venom polypeptide, preparation and application thereof
  • Novel snake venom polypeptide, preparation and application thereof

Examples

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preparation example

[0048] method:

[0049] 1) 20 mg of the crude venom of Agkistrodon acutus was fully dissolved in 500 μl of ultrapure water, centrifuged at 5000 rpm for 5 min at 4°C to remove insoluble substances, and the supernatant was taken for later use.

[0050] 2) Use a diameter of 5 μm and a pore size of 300 Reversed-phase silica gel C18 granules were used as a stationary phase filler to make a high-performance liquid chromatography C18 reverse-phase preparative column (20×300mm). The chromatographic mobile phase is 0.1% trifluoroacetic acid and acetonitrile, the flow rate is 7ml / min, and the detection wavelength is 210nm. On the HPLC chromatography system, 0.1% trifluoroacetic acid + acetonitrile is used as the mobile phase, and 0.1% trifluoroacetic acid is eluted The gradient is from 100% to 20%, the gradient of acetonitrile elution is from 0 to 80%, the elution time is 120min, the separation temperature is 25°C, and the components with absorbance of 0.5-2.0 are collected at the elu...

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Abstract

The invention provides a novel snake venom polypeptide, which is extracted from the coarse venom of pit vipers and has molecular weight of 7554Da. The sequence of 1-15 amino acids at the N terminal is shown in formula (I), wherein E is glutamic acid; A is lactamic acid; D is aspartic acid; N is asparagines; C is cystine; and N represents an amino terminal. The snake venom polypeptide has high anti-tumor activity, and is applicable in preparing anti-tumor pharmaceuticals. The formula (I) is N-EAEEDEDDDAAAANC.

Description

field of invention [0001] The invention belongs to the field of biochemistry, and in particular relates to a new snake venom polypeptide. Background technique [0002] Snake venom is a substance secreted by snake glands of venomous snakes. It has various pharmacological effects, such as analgesia, hemostasis, inhibition of thrombosis, and antitumor. Some snake venom preparations have been used clinically. Snake venom is the most complex type of animal poison. Each snake venom contains at least 20 kinds of active ingredients, mainly including various enzymes and poisonous proteins. The body produces serious toxic and side effects, such as cardiotoxicity and neurotoxicity, which are the main reasons for limiting the application of snake venom preparations in clinical treatment. [0003] With the rapid development of various related disciplines, the level of research on snake venom is gradually deepening from the level of crude venom to the level of separation and purification...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/20A61K38/17A61P35/00
Inventor 王广发吴少瑜张嘉杰饶进军徐伟吕琳吴曙光
Owner SOUTHERN MEDICAL UNIVERSITY
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